The laboratory diagnosis of herpes simplex virus infections
Direct examination of vesicle fluid or other clinical material by electron microscopy for the diagnosis of HSV is limited by the fact that viral morphology cannot be used to distinguish HSV from other herpes viruses (eg, varicella zoster virus) (13).
Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care ...
Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing.
Light Microscopy, Culture, Molecular, and Serologic Methods for ...
2013年10月16日 · In this article we review the different methods for detection and typing of HSV, including light microscopy, culture, serology, and nucleic acid-based tests. We discuss the strengths and weaknesses of each method for diagnosing HSV infection, cite performance characteristics, and review appropriate clinical uses.
Structural analysis of herpes simplex virus by optical super
2015年1月22日 · Here we use super-resolution imaging by direct stochastic optical reconstruction microscopy (d STORM) in combination with a model-based analysis of single-molecule localization data, to determine...
Herpes virus infection pathology - DermNet
Herpes simplex, varicella and herpes zoster infections show identical histology. Clinical correlation or immunohistochemistry and/or viral culture or PCR (of an appropriate swab or tissue specimen) is required to differentiate these viral infections. The histology of herpes infections is …
Light microscopy, culture, molecular, and serologic methods …
In this article we review the different methods for detection and typing of HSV, including light microscopy, culture, serology, and nucleic acid-based tests. We discuss the strengths and weaknesses of each method for diagnosing HSV infection, cite performance characteristics, and review appropriate clinical uses.
Structure of the herpes simplex virus type 2 C-capsid with capsid ...
2018年9月10日 · Here, we report the cryo-electron microscopy structure of the HSV-2 C-capsid with capsid-vertex-specific component (CVSC) that was determined at 3.75 Å using a block-based reconstruction...
Cryo-EM structure of a herpesvirus capsid at 3.1 Å - Science
2018年4月6日 · Using cryo–electron microscopy (cryo-EM) with an optimized image reconstruction strategy, we report the herpes simplex virus type 2 (HSV-2) capsid structure at 3.1 angstroms, which is built up of about 3000 proteins organized into three types of hexons (central, peripentonal, and edge), pentons, and triplexes.
Structure of the herpes simplex virus 1 capsid with ... - Science
2018年4月6日 · Dai and Zhou used electron microscopy to determine a high-resolution structure of the HSV-1 capsid bound to the tegument proteins that occupy the space between the capsid and the nuclear envelope. The structure suggests how these components may play a …
Cryo-EM structures of herpes simplex virus type 1 portal vertex …
2019年5月29日 · To better define the structural basis of genome packaging and organization in herpes simplex virus type 1 (HSV-1), we developed sequential localized classification and symmetry relaxation...