HTLV I/II DNA, Qualitative, Real-Time PCR - HTLV-I/II DNA PCR is a highly specific and sensitive method used to detect HTLV-I/II proviral DNA in clinical specimens. In addition, the assay can also differentiate between HTLV-I and HTLV-II infected individuals.

We propose a new testing algorithm for the laboratory diagnosis of HTLV infection, which includes HTLV specific mqRT-PCR for resolving HTLV serological results. Furthermore, quantitation of PVL load by real-time PCR may be useful in assessing the link between infection and disease, and in monitoring …

HTLV I/II DNA, Qualitative Real-Time PCR. Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed.

We developed and validated two in-house multiplex quantitative real-time PCR for HTLV-1/-2 (mqPCR_HTLV) assays, targeting the pol and tax genes, for the simultaneous identification of HTLV-1, HTLV-2, and the albumin reference gene. The robustness of the assays was evaluated on two platforms using seven commercial master mix formulations.

Human T-Lymphotropic Virus (HTLV) I/II Quantitative Real-time PCR assay detects and distinguishes HTLV-1 virus, which can cause adult T-cell leukemia/lymphoma and other serious conditions, and HTLV-2 virus.

2023年1月17日 · HTLV-1 is the first RNA retrovirus found to be mainly transmitted by mother-to-child, sexual contact and injecting drug users [1, 2]. It also can be transmitted by the transfusion of infected blood components, liver, kidney and lung transplantation [3].

2024年12月1日 · In conclusion, the proposed rtPCR-HRM method allows for the detection and discrimination of HTLV-1 and HTLV-2, eliminating the need for post-PCR processing steps such as agarose gel electrophoresis and ethidium bromide staining. As a result, a fast, cost-effective assay simplifies the n-PCR process and is more accessible than immunoassays.

The human T-cell leukemia virus type 1 (HTLV-1), a retrovirus, integrates into host DNA and causes adult T-cell leukemia/lymphoma (ATL) in some individuals. Two types of defective proviruses, Type 1 and Type 2, are often observed in ATL cells. Here, we developed a 3-plex digital PCR (dPCR) method to …

5 天之前 · The human T-cell lymphotropic virus type 1 (HTLV-1) is a retrovirus that causes HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). ... for HTLV-1 tax. The second round of PCR was performed under the same conditions using 2 µl of the products from the first PCR round. The amplification cycle consisted of enzyme activation at ...

5 天之前 · Human T lymphotropic virus type 1 (HTLV-1) chronic infection is due to the mitotic proliferation of infected CD4+ T cells, where the proviral DNA is integrated in its host DNA. ... We present an IS assay that solely relies on PCR and Sanger sequencing, which allowed HTLV-1 IS determination in four patients with various HTLV-1-associated ...