Polarized actin and VE-cadherin dynamics regulate junctional
2017年12月20日 · Here, we show that VEGF-induced polarized cell elongation increases cell perimeter and decreases the relative VE-cadherin concentration at junctions, triggering …
Quantitative image analysis of F-actin in endothelial cells
The digitized images of individual human umbilical vein endothelial cells (HUVEC) were imported into a F-actin image analysis program (FAAP) and converted to layers, each one pixel thick.
Transient and Prolonged Increase in Endothelial Permeability …
1998年11月30日 · Immunocytochemical staining of actin (A through D) and vinculin (E through H) in HUVEC. ECs were preincubated for 1 hour with 30 μg/mL genistein and stimulated with 1 …
Lipopolysaccharide induces vascular endothelial cell pyroptosis …
2021年5月1日 · RCN2 overexpression rescued the inhibitory effect of SP1 inhibitor on HUVEC pyroptosis induced by LPS (all p < 0.05). These findings suggested that the activation of the …
Subendothelial stiffness alters endothelial cell traction force ...
2019年12月3日 · In this study, we compared the responses of two different types of ECs to growth on stiff versus soft hydrogel substrates, primary human umbilical vein endothelial cells …
钙库操纵性钙内流参与调节ox-LDL诱导的血管内皮细胞通透性改变
[摘要] 目的 利用体外培养的人脐静脉血管内皮细胞 (human umbilical vein endothelial cells，HUVECs)，探讨钙库操纵性钙内流 (store-operated calcium entry，SOCE)在氧化低密 …
Actin cytoskeletal isoforms in human endothelial cells in vitro ...
The microfilamentous actin component of the cytoskeleton is crucial to endothelial angiogenesis and vascular permeability. Differences in actin cytoskeletal profiles in cultured human …
Thrombin-Induced Increase of F-Actin in Human Umbilical Vein ...
1994年1月1日 · We have studied the actin cytoskeleton in human umbilical vein endothelial cells (HUVEC) growing on tissue culture plastic coverslips or 0.4-μm pore-size polycarbonate …
HUVEC actin cytoskeleton remodeling following treatment with …
The effect of β-escin sodium up to 40 µg/mL has been shown to inhibit proliferation and migration but also to initiate apoptosis at the maximum concentration in HUVEC and human umbilical …
Immunofluorescence images of HUVEC monolayers after 48 h
HUVECs exposed to the UND waveform showed a loss of peripheral actin bands and a greatly increased number and thickness of F-actin ...