2019年4月1日 · A first step towards the direction of serum-free and chemically defined medium for vascular constructs was reported by Huttala et al. demonstrating the formation of EC networks of human umbilical...

2025年1月15日 · In this work, we introduced exosomes rich in VEGF derived from human umbilical vein endothelial cells (HUVECs) into the surface of Glut-BP to increase its capacity for endothelialization. Compared with Glut-BP, the exosome-coated BHVs showed good biocompatibility, improved endothelialization and reduced thrombosis and calcification.

2024年7月2日 · HucMSCs and human umbilical vein endothelial cells (HUVEC) were isolated and cultured from human umbilical cord and characterized. Lentiviral vectors expressing miRNA-25-3p were transfected into...

2014年2月19日 · Human umbilical vein endothelial cells (HUVECs) were successfully entrapped in polyethylene oxide (PEO) core /polycaprolactone (PCL) shell electrospun fibers thus creating a “bioactive fiber.” The viability and release of biomolecules from the entrapped cells in the bioactive fibers were characterized.

2022年11月1日 · Herein, we showed that EFs induced human umbilical vein endothelial cells (HUVEC) migration toward the cathode with enhanced autophagy. Genetic ablation of autophagy by silencing the autophagy-related gene (Atg) 5 abolished the EFs-directed migration of HUVEC, indicating that autophagy is definitely required for EFs-guided migration of cells.

2022年1月1日 · Co-culture of human umbilical vein ECs (HUVEC) with MSC increased mRNA expression levels of uPAR, MMP14, VEGFR2, TGFβ1, integrin β3 and Notch pathway components (Notch1 receptor and ligands: Dll1, Dll4, Jag1) in HUVECs and uPA, uPAR, TGFβ1, integrin β3, Jag1, Notch3 receptor in MSC.

We found that vasostatin could inhibit the cell viability of HUVEC and induce cell apoptosis through mitochondrial pathways via activation of caspase-3 under oxygen deprivation conditions. Meanwhile, vasostatin also inhibited vascular endothelial growth factor-induced proliferation and tube formation of HUVEC.

2020年5月12日 · HUVEC were reliant on glycolysis and FAO, and inhibition of either pathway disrupted cell growth and proliferation. VEGF-A was a potent inducer of glycolysis in tubulogenic HUVEC, while FAO was...

海星生物提供的脐静脉内皮细胞系(HUVEC细胞系)取自自然分娩、健康的新生儿脐带组织经SV40T转化,具有良好的传代能力及性状表现。 胰蛋白酶-EDTA消化液(0.25%)含酚红(胰酶)在37°C消化1-2min。 注:不同品牌胰酶不同细胞密度消化时间略有区别,以大部分细胞变圆脱落为准。 细胞培养中会有少量黑色颗粒及死细胞碎片的产生,属正常现象,可通过换液将其去除。 使用海星配套完培,按照1:2传代,收货后承诺能再传5代。 该细胞为有限细胞系,建议收到后尽快进行实验, …

2023年5月25日 · 在内皮细胞生长培养基中培养人脐静脉内皮细胞（HUVECs）：内皮细胞基础培养基中添加 1 μg/mL 氢化可的松和 1 ng/mL 表皮生长因子，10% 胎牛血清，青霉素/链霉素，在 37 ℃，5% CO2 的条件下培养。 2. 当融合 80% 时，每次传代以 1:3 的比例分裂细胞。 使用传代 2~5 次的细胞，这些细胞对生长因子的反应没有明显差异。 传代 6 次之后，细胞开始衰老，不再在凝胶化的基底基质上形成管状细胞。 二、转染. 1. 当 HUVECs 融合度达到 70% 时，用所需基因转 …