
Characterization of human leukocyte-HUVEC adhesion: Effect …
2017年4月1日 · HUVEC expressed under resting conditions the adhesion molecules ICAM-1, VCAM-1 and E-selectin and their expression was upregulated by stimulation with TNF-α (0.1–10 ng/ml) as well as with LPS (1 μg/ml). SEM analysis showed that stimulation with both stimuli profoundly affect cell morphology.
Transient and Prolonged Increase in Endothelial Permeability …
1998年11月30日 · In HUVEC, the addition of histamine (3×10 −6 mol/L) induced a rapid decrease in the real-time TEER, which was maximal after 1 minute (72±7% compared with basal level; 5 different cultures in triplicate) and lasted for <5 minutes (Figure 1A).
Characterization of human leukocyte-HUVEC adhesion: Effect …
We investigated by the means of flow cytometry and microscopic techniques (both optical microscopy [OM] and scanning electron microscopy [SEM]), the granulocyte-endothelium adhesion and the role of sample manipulation.
A bioprosthetic heart valve modified by HUVEC-exosomes with …
2025年1月15日 · In this work, we introduced exosomes rich in VEGF derived from human umbilical vein endothelial cells (HUVECs) into the surface of Glut-BP to increase its capacity for endothelialization. Compared with Glut-BP, the exosome-coated BHVs showed good biocompatibility, improved endothelialization and reduced thrombosis and calcification.
Cobblestone HUVECs: A human model system for studying …
2011年12月1日 · To screen large areas of HUVEC cell surfaces for protruding cilia, SEM was performed (Fig. 3) on cultured HUVECs and on the luminal endothelial surface of the umbilical veins. Since the timescale immunofluorescence experiments showed that about 60% of the cultured endothelial cells form a primary cilium after being cobblestone for 10 days, many ...
人白细胞-HUVEC粘附的表征:细胞制备方法的影响。,Journal of …
2017年2月9日 · sem分析表明,两种刺激均能深刻影响细胞形态。 流式细胞术评估细胞黏附性表明,两种制剂中细胞黏附于HUVEC单层的能力差异很大,在所有细胞亚群中FB的黏附性最低。
Scanning Electron Microscopy (SEM) imaging of HUVEC
Scanning Electron Microscopy (SEM) imaging of HUVEC cells attachment on the surface of a tubular SIS-based vascular graft subjected to the initial static growing...
特殊细胞培养——人脐静脉内皮细胞(HUV-EC-C) - 知乎
目前市面上主要有两种人脐静脉内皮细胞,一种是原代细胞,英文缩写为huvec,另一种是有限 传代细胞系 ,英文缩写为huv-ec-c。 该细胞可用于心血管疾病的研究,常见于研究低氧、炎症、 氧化应激 、感染反应以及肿瘤相关血管生成等应用中。
The Osteogenic Differentiation of Human Bone Marrow MSCs on HUVEC …
Here we report that ECM derived from human umbilical vein endothelial cells (HUVEC) promoted osteogenic differentiation of human bone marrow mesenchymal stem cells (hMSC). We first produced an HUVEC-derived ECM on a three-dimensional (3D) beta-tricalcium phosphate (β-TCP) scaffold by HUVEC seeding, incubation, and decellularization.
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人脐静脉血管内皮细胞永生化[HUVEC]
人脐静脉内皮细胞 (huvec) 是从脐带静脉中分离出来的原代细胞建立的转化细胞系。 HUVEC形态呈现为典型的鹅卵石样排列,已证明在体外可传至40代。 内皮标记vWF、CD31、CD34阳