Bone marrow cells of galine transformed by avian leukemia virus. Culture Medium: RPMI-1640 medium modified with 4500 mg/L glucose and 10% of fetal bovine serum. Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with PBS without calcium and magnesium to remove all traces of serum that contains trypsin inhibitor.

Breed/subspecies: White Leghorn SPAFAS. Chicken hematopoietic cells transformed by seven strains of defective avian leukemia viruses display three distinct phenotypes of differentiation.

HD11 cells are a macrophage-like immortalized cell line derived from chicken bone marrow and transformed with the avian myelocytomatosis type MC29 virus [26].

2022年5月3日 · HD11 is a chicken macrophage-like cell line that was derived from chicken hematopoietic cells after in vitro transformation with the avian myelocytomatosis type MC29 virus (Beug et al., 1979). HD11 cells were maintained in the Iscove Modified Dulbecco Media (IMDM, Gibco) medium supplemented with 10% fetal bovine serum (FBS) at 39°C in a ...

2021年1月28日 · In this study, HD11 chicken macrophage cells and chicken peripheral blood mononuclear cell-derived macrophages (PBMCs-Mφ) were infected with IBV M41 strain, and the dynamic changes of macrophage functions and gene expressions of related proteins/mediators in innate and acquired immunity, inflammation, autophagy and apoptosis were ...

2017年4月1日 · The HD11 cell line is a macrophage-like cell created by transforming chicken hematopoietic cells with avian myelocytomatosis type MC29 virus (Beug et al., 1979; Leutz et al., 1988). The HD11 cells have been shown to phagocytize Salmonella Typhimurium and S. Enteritidis (with an MOI of 25) reducing the inoculum by approximately 2 log CFU/mL in 3 ...

2020年11月1日 · Although macrophage cell line (HD11) have been shown to take up and kill avian pathogenic E. coli (APEC) (Peng et al. 2018), the role of primary macrophages in controlling APEC is less studied due to the lack of standardization of culturing and …

hd11 鸡巨噬细胞 产品基本信息 细胞名称：hd11，鸡巨噬细胞 种属来源：鸡 组织来源：骨髓 细胞形态：上皮细胞样 生长特性：贴壁生长 培养基:： 1640+10%fbs+ps 生长条件：气相：95%空气+5%二氧化碳；温度：37℃ 传代方法：1:2至1:3，每周2-3次

2021年2月4日 · For this, we used chicken macrophage-like cell line HD11 cultures infected in vitro with purified E. tenella sporozoites. Cultures were harvested between 2 and 72 h post-infection and mRNA was extracted and sequenced. Dual RNA-Seq analysis showed clear patterns of altered expression for both parasite and host genes during infection.

HD11鸡巨噬细胞. 货号：IM-C072. 规格：1x10 6 cells/T25或1mL冻存管 . 形态：上皮细胞样，贴壁生长. 培养基：1640+10%FBS+PS. 培养环境：气相：95%空气+5%二氧化碳;温度：37℃ 价格： 4500元