1998年10月15日 · Heat shock proteins (Hsp) of four Rickettsia species, three Bartonella species, two Ehrlichia species, Orientia tsutsugamushi and seventeen other eubacterial species were characterized by the enhanced chemiluminescence Western blotting (WB) technique with antibodies raised against recombinant Hsp from Escherichia coli and purified GroES from R ...

2016年3月18日 · Heat shock protein 70 (HSP70) expression by Western blot. Fig 1B shows a representative western blot of plasma HSP70 and β-actin protein of 132 patients with essential hypertension and 132 controls. A significant difference for the HSP70 plasma protein expression level was observed in patients (1.43 ± 0.02) and control groups (1.11 ± 0.01 ...

In this study, we aimed to evaluate the serodiagnostic potential of two heat shock proteins (Hsps) i.e. Hsp70 and Hsp83 in combination by ELISA and western blotting in mouse model. Parasite proteins were separated by SDS-PAGE and transferred to PVDF membranes which were further incubated with serum samples of infected mice for visualizing ...

Objective: To assess the validity of the Western blot immunoassay for heat shock protein-70 (hsp-70) for diagnosis of autoimmune inner ear disease. Study design: Retrospective study of 53 patients affected by sudden deafness (n = 19), idiopathic progressive sensorineural hearing loss (n = 24), and Meniere's disease (n = 10) who were treated ...

2023年11月17日 · Therefore, in this study, we used Caco-2 cells to establish a heat stress model and vitamin C as a positive control to investigate the regulatory effects of vitexin on heat-stress-induced apoptosis and the related mechanisms using Cell Counting Kit-8, flow cytometry, real-time quantitative polymerase chain reaction, and Western blot.

Objectives: Our preliminary study investigated the role of nonspecific immunologic tests and immunoassay for heat shock protein 70 (HSP-70) in supporting the possibility of an autoimmune inner ear process determining idiopathic tinnitus.

Heat shock proteins (HSPs) play an essential role in protecting proteins from denaturation and are implicated in diverse pathophysiological conditions like cardiovascular diseases, cancer, infections, and neurodegenerative diseases.

2015年5月1日 · Heat shock proteins (HSPs) are an evolutionary family of proteins that act as molecular chaperones. According to their size they have been classified into the following families; HSP90, HSP70, HSP60, HSP40 and HSP27. They prevent the formation of nonspecific protein aggregates and they assist proteins in the acquisition of their normal ...

2022年9月10日 · Using Western blot analysis, we demonstrated the overproduction of MaltHSP70-2 protein in the whole body and reproductive tissues of adults under heat stress treatment. Immunohistochemical assay of MaltHSP70-2 protein in testis specifically showed that primary spermatocyte was the leading site for overproduction of MaltHSP70-2 protein rather ...

(D–K) Display quantified protein levels for HSPA1, HSPA2, HSPD1, HSPB1, HSPB5, HSPB7, HSPA4, and HSP90, respectively. Data were statistically analyzed by unpaired two-tailed t-test. Dashed line...