Hektoen enteric agar is currently applicable as a direct and indirect plating medium to recover gastrointestinal pathogens, such as Salmonella and Shigella, from food, water, and fecal …

Hektoen enteric agar (HEK, HE or HEA) is a selective and differential agar [1] primarily used to recover Salmonella and Shigella from patient specimens. HEA contains indicators of lactose fermentation and hydrogen sulfide production; as well as inhibitors to prevent the growth of Gram-positive bacteria.

2022年1月4日 · Hektoen Enteric Agar is a selective and differential medium designed to isolate and differentiate members of the species Salmonella and Shigella from other Enterobacteriaceae. The medium was introduced in 1968 by Sylvia King and William I. Metzger.

HE agar is currently used as both a direct and indirect plating medium for fecal specimens to enhance the recovery of species of Salmonella andShigella from mixed normal fecal flora. Modification and refinement by later microbiologists of …

Chart showing surface area, seeding density, cells at confluency, and volumes of Versene, trypsin and medium for various culture dishes, plates and flasks.

2023年9月20日 · Hektoen Enteric Agar (HE Agar) is a selective and differential agar medium used to isolate and differentiate enteric pathogens, particularly Salmonella and Shigella. It was developed in 1967 by King and Metzger, and is named after the Hektoen Institute in Chicago, where it was developed.

2024年5月16日 · Hektoen Enteric Agar (HE agar) is a selective and differential medium used in clinical microbiology labs to aid in the recovery and identification of Salmonella and Shigella species from clinical specimens, particularly fecal samples. It was introduced in 1968 by Sylvia King and William I. Metzger at the Hektoen Institute in Chicago.

HEK cell behavior is important to understand in order to recognize the health of the cells and to appreciate the procedures that are used in the lab. Healthy HEK-293 cells grow on the surface of the cell culture plate. They adhere to the plate by proteins on the cell surface.

Hektoen Enteric agar is a selective and differential medium for the isolation and differentiation of pathogens from clinical samples. Selectivity : The presence of bile salts and dyes inhibits most Gram-positive organisms, which only allows Gram-negative bacilli to develop on agar.

Remove plate from incubator. Add 8mL fresh media to stop rxn. Gently pipette up and down 8-10 times to get single cells. These cells adhere poorly to standard tissue culture flasks. Attachment can be enhanced by using Corning CellBIND® flasks.