The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen. This cell line is competent to replicate vectors carrying the SV40 region of replication. It gives high titers when used to produce retroviruses.

HEK293T is a popular derivative of the HEK293 cell line. Scientists developed these cells from the original human embryonic kidney 293 cells by transfecting them with SV40 large T antigen, while they established the immortalized HEK293 cells by transforming and culturing human embryonic kidney cells with sheared human adenovirus 5 DNA fragments.

HEK293T cells, with their higher transfection efficiency, often yield higher protein expression levels compared to HEK293 cells. This attribute makes HEK293T cells a preferred choice for researchers aiming to produce large quantities of proteins for biochemical and structural studies.

1999年9月14日 · 293T/17 [HEK 293T/17] is a cell line exhibiting epithelial morphology that was isolated from human embryo kidney tissue.

In retroviral vector production, HEK293T is used due to the expression of the SV40 large T-antigen in the cell line. Expression of the T-antigen allows plasmids which carry the SV40 origin of replication to undergo replication when transfected into the cell (DuBridge et al., 1987).

2024年11月12日 · HEK293T is a subclone of the HEK293 cell line, modified to express the SV40 T antigen. The expressed SV40T antigen enables the amplification of foreign expression vectors, ultimately increasing the expression of target genes.

Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations. Global miRNA expression profiling identifies miR-1290 as novel potential …

Human embryonic kidney 293T (HEK293T) cells are used in various biological experiments and researches. In this study, we investigated the effect of cell culture environments on morphological and functional properties of HEK293T cells.

Here, we provide a step-by-step protocol for cell culture, plasmid transfection in HEK293T, and adenoviral infection in C2C12 cells for gene overexpression in vitro, using MG53 as an example. This protocol enables sufficient and efficient gene expression for …

2014年9月3日 · First among these derived lines, we analysed 293T, which expresses a temperature-sensitive allele of the SV40 T antigen 8, 9. This enables the amplification of vectors containing the SV40...