氯喹可以通过抑制溶酶体功能（比如升高它的pH值），抑制LC3-II的降解；巴佛洛霉素A1则是一种强效V-ATPase抑制剂，阻断溶酶体依赖的降解。 在“堵漏”的前提下，根据一段时间内LC3-II的积聚程度，LC3-II/ LC3-I的比值，判断自噬的改变。 通常情况下认为LC3-II/ LC3-I大于1即认为有自噬的发生。 目前的LC3抗体所检测的抗原表位都位于LC3的N端，由于PE基团结合导致N端区域的构象变化，使得抗体对LC3-II的亲和性更高，LC3-II/ LC3-I的比值存在较大的误差。 LC3-II条带显 …

2017年4月5日 · FKBP 8 (also called FKBP 38) is a novel mitophagy receptor that selectively recruits LC 3A to damaged mitochondria to mediate Parkin‐independent degradation of mitochondria by autophagy. The anti‐apoptotic FKBP 8 itself escapes degradation to ensure cell survival. FKBP 8 binds to LC 3A via an N‐terminal LIR motif.

2023年11月14日 · Activity-based protein profiling (ABPP) based evaluations reveal that a derivative molecule DC-LC3in-D5 exhibits a potent covalent reactivity and selectivity to LC3A/B in HeLa cells. 2023-11-14: Updated project metadata.

2019年1月24日 · Atg8 localization was assessed using HA-tagged variants of all six mammalian Atg8s (LC3A, LC3B, LC3C, GBRP, GBRPL1 and GBRPL2), to avoid the functional impairment associated with GFP-tagged...

In a yeast two-hybrid screen, we identified FKBP8 as an ATG8-interacting protein. Here, we map an N-terminal LC3-interacting region (LIR) motif in FKBP8 that binds strongly to LC3A both in vitro and in vivo FKBP8 efficiently recruits lipidated LC3A to …

2006年1月6日 · In present work, we cloned LC3A and LC3B, two novel variants of rat LC3. The expression patterns of LC3, LC3A, and LC3B were different in 12 normal rat adult tissues. LC3A and LC3B undergo a characteristic C-terminal cleavage …

In the current study we examine, after extensively validating antibody specificity, the expression in parallel of the three LC3A, B and C proteins in human cancer cell lines, showing distinct patterns of sub-cellular localization, suggesting a distinct biological role of these sister proteins.

Novus 提供即用型 HeLa 氯喹处理/未处理细胞裂解物 ( NBP2-49689 ) 和 Neuro2a 氯喹处理/未处理细胞裂解物 ( NBP2-49688 )，它们是 LC3 WB 检测的强烈推荐的阳 性对照。

Activity-based protein profiling (ABPP) based evaluations reveal that a derivative molecule DC-LC3in-D5 exhibits a potent covalent reactivity and selectivity to LC3A/B in HeLa cells.

图 4：使用经过氯喹（CQ）处理的 Hela 细胞蛋白裂解物（LC3 Control Cell Extracts #11972，为阳性对照），LC3A/B 抗体（12741#）进行检测