Recently, the Ikaros family member, Helios, was reported as a marker to discriminate naturally occurring, thymic-derived Tregs from those peripherally induced from naïve CD4+ T cells. We investigated Helios expression in murine and human T cells under resting or activating conditions, using well-characterized molecules of naïve/effector ...

In this study, we characterized by flow cytometry and transcriptomic analyses human HELIOS + CD8 T cells. These T cells primarily consist of memory cells and constitute approximately 21% of blood CD8 T cells.

2023年12月22日 · 与内存 helios 相比- t-bet高的cd8 t 细胞表现出强大的效应功能，即记忆 helios + t-bet高的cd8 t 细胞产生较低量的 ifn-γ 和 tnf-α，并且具有较低的细胞毒性潜力。

2011年8月30日 · We found that Helios-negative T cells are enriched for naïve T cell phenotypes and vice versa. Moreover, Helios can be induced during T cell activation and proliferation, but regresses in the same cells under resting conditions. We demonstrated comparable findings using human and murine CD4+Foxp3+ Tregs, as well as in CD4+ and CD8+ T cells.

2023年5月1日 · We have demonstrated that ~10–40% of CD8 + T cells from normal donors express Helios. Expression of Helios in CD8 + appears to be downregulated with TCR stimulation alone but is maintained in the presence of excess IL-2 signaling. Thus far, extensive flow cytometry studies have failed to show a correlation between Helios expression and any ...

2022年5月15日 · We found that co-expression of the transcription factor Helios and the aging-related marker TIGIT identifies CD8 + T cells that fail to proliferate and show impaired induction of activation markers CD69 and CD25 in response to stimulation in vitro.

2023年12月22日 · Our findings emphasize that HELIOS is expressed across various CD8 T cell populations, highlighting its significance beyond its role as a transcription factor for Treg or exhausted murine CD8 T cells. The significance of the connection between HELIOS and HLA-E restriction is yet to be understood.

We found that Helios- T cells (Tregs, CD4+Foxp3- cells, CD8 cells) in mice and humans were enriched for naïve phenotypes, while Helios+ cells showed more mature phenotypic characteristics. Since iTreg require TCR-induced activation for their development, these data were not consistent with the concept that naive nTregs are primarily Helios+ ...

2022年6月16日 · We observed that all MRL animals showed a dramatically reduced population of CD8 + Tregs and a greater Helios downregulation on diseased mice. Helios induction was detected preferentially on CD8 + T cells from OT-I mice co-cultured with tolDCs from C57BL/6 but not in MRL animals.

2022年3月8日 · In this work, we find that CD8+ T cells expressing inhibitory killer cell immunoglobulin-like receptors (KIRs) are the human equivalent of Ly49+CD8+ regulatory T cells in mice and are increased in ...