2024年2月28日 · In this study, we show that XBP1s, the major downstream effector of IRE1α, is required for HCC cell survival both in vitro and in vivo. Mechanistically, XBP1s transactivates LEF1, a key co-factor...

2020年11月20日 · Genetic deletion of XBP1 has multiple consequences: some resulting from the loss of the transcription factor per se, and others related to compensatory activation of IRE1α. The objective of the...

2018年4月15日 · 结果表明单宁酸能够联合顺铂增强肝癌 HepG2 细胞内质网应激 IRE1-XBP1 通路的激活水平，提示 IRE1-XBP1 通路可能是单宁酸和顺铂协同抗肝癌 HepG2 细胞的分子机制之一。

XBP1 pathway activation increased in Huh7-Ntcp and HepG2 cells treated with bile acids, 6α-ethyl-chenodeoxycholic acid (6-ECDCA) or GW4064. This effect decreased with FXR knockdown and treatment with the FXR inhibitor guggulsterone. FXR agonists increased XBP1 splicing and phosphorylated IRE1α (p-IRE1α) expression.

In this study, we show that XBP1s, the major downstream effector of α IRE1 , is required for HCC cell survival both in vitro and in vivo. Mechanistically, XBP1s transactivates LEF1, a key co-factor...

2017年4月1日 · HepG2 cells transfected with one of two different siRNAs directed to knock-down spliced XBP1 or with control siRNA were subsequently treated with IL6. We observed that XBP1 knock down had a slight effect on Tyr705-Stat3 phosphorylation, while it efficiently inhibited phosphorylation at Ser727 in proportion to the intensity of XBP1 suppression ...

Xbp1 mRNA splicing, JNK activation, and IRE1α-dependent RNA decay consistently correlate with increased activity of IRE1α in fructose-induced liver injury in hepatocyte-specific XBP1 KO mice. Additionally, there is evident activation of eIF2α, accompanied by the upregulation of pro-apoptotic molecules such as CHOP, BIM, and PUMA.

2022年11月11日 · In vitro HepG2 cells or XBP1 knockout AML12 hepatocytes were stimulated with metformin, palmitic acid or small interfering RNA inducing XBP1 knockdown, or dominant-negative mutant AMPK plasmid. Metformin treatment …

In the present study the hepatitis B virus X antigen binding protein 1 (XBP1) was cloned by inducing its expression, and its subcellular localization and function were examined. Total RNA was extracted from HepG2 cells and XBP1 was amplified using reverse transcription polymerase chain reaction (RT- …

2007年3月1日 · In this study, the probable roles of HBx in GRP78 and XBP1 activation in transfected Hep3B and HepG2 cells were first examined by using a dual luciferase assay. The two transcriptional programs of UPR, the ATF6 and IRE1-XBP1 pathways, were also investigated in Hep3B cells with transient or stable expression of HBx.