The AML12 (alpha mouse liver 12) cell line was established from hepatocytes from a mouse (CD1 strain, line MT42) transgenic for human TGF alpha. By electron microscopy, these cells exhibit typical hepatocyte features such as peroxisomes and bile canalicular like structure.

Hepatic stellate cells (HSC), also known as perisinusoidal cells or Ito cells (earlier lipocytes or fat-storing cells), are pericytes found in the perisinusoidal space of the liver, also known as the space of Disse (a small area between the sinusoids and hepatocytes).

Hepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes.

We established and characterised two human HSC cell lines termed LX-1 and LX-2, and compared their features with those of primary human stellate cells. Methods and results: LX-1 and LX-2 were generated by either SV40 T antigen immortalisation (LX-1) or spontaneous immortalisation in low serum conditions (LX-2).

We established and characterised two human HSC cell lines termed LX-1 and LX-2, and compared their features with those of primary human stellate cells. Methods and results: LX-1 and LX-2 were generated by either SV40 T antigen immortalisation (LX-1) or spontaneous immortalisation in low serum conditions (LX-2).

Cell Culture. Human hepatic stellate cell line LX-2 , Wi-38 (clone VA-13, subline 2RA) (#CCL-75.1, ATCC, Manassas, VA, USA) , HepG2 , and Hep3B were cultured in HEPES-buffered Dulbecco's modified Eagle medium (DMEM) (Lonza BioWhittaker, Verviers, Belgium) supplemented with 4 mmol/L L-glutamine (Lonza), 100 IU/ml Penicillin/100 µg/ml ...

Hepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes.

3 天之前 · Hepatic stellate cells (HSCs) are activated with chronic liver injury and transdifferentiate into myofibroblasts, which produce excessive extracellular matrices that form the fibrotic scar. While ...

2022年10月5日 · Here we interrogated functions of hepatic stellate cells (HSCs), the main source of liver fibroblasts 3, during hepatocarcinogenesis. Genetic depletion, activation or inhibition of HSCs in...

5 天之前 · Mice were injected with 5 × 10 4 B16F10 melanoma cells (ATCC, Cat #CRL-6475) into the flank of the mouse ... X. K. et al. Focal adhesion kinase regulates hepatic stellate cell activation and ...