Properties and biotechnological applications of halohydrin ...
2012年11月1日 · Halohydrin dehalogenases (HHDHs) are lyases that catalyze the cleavage of carbon–halogen bond of halohydrins. They also can catalyze the reverse reaction in the …
Recent advances on halohydrin dehalogenases—from enzyme …
These enzymes, also called halohydrin dehalogenases (HHDHs), haloalcohol dehalogenases, or halohydrin hydrogen-halide-lyases, belong to the enzyme class of lyases (EC 4.5.1.-) and …
A high-throughput screening assay for the directed evolution …
2020年3月10日 · To use directed evolution to unleash the latent potential of HHDHs for the synthesis of β-substituted alcohols, we report a high-throughput assay for screening HHDHs …
Intact-cell detection of halohydrin dehalogenase activity and ...
2024年11月1日 · The discovery of novel HHDHs from microorganisms in niche environments including the sea requires in-situ analysis of HHDH activity. Owing to its high practical value, …
Rational design of halohydrin dehalogenase for efficient chiral ...
2020年9月15日 · Halohydrin dehalogenases (HHDHs) represent as important biocatalysts for the production of valuable chiral epoxides and β-substituted alcohols. The HHDH from A. …
HheG，一种对环氧化物具有活性的卤代醇脱卤酶,ACS Catalysis - X …
2017年9月13日 · 卤代醇脱卤化酶（HHDH）具有混杂的环氧化物开环活性和一组带负电荷的亲核试剂，因此具有生物技术意义，能够形成C–C，C–N或C–O键。 HHDH特异性序列基序的最 …
磁性生物炭中的卤代醇脱卤酶固定化可持续卤代烃生物降解和生物 …
关于卤代醇脱卤酶 (HHDH) 在生物转化和生物降解方面显示出巨大潜力的事实，开发适用于这些应用的固定化 HHDH 将非常重要。 在此，制备了功能化磁性生物炭，用于高效的酶固定化、易 …
卤代醇脱卤酶催化的二取代环氧化物的选择性开环,ChemCatChem
2019年3月15日 · 卤代醇脱卤酶（HHDHs）是用于合成β-取代醇的有价值的生物催化剂，基于它们与许多小阴离子亲核试剂的环氧化物开环活性。 在试图进一步拓宽这些酶底物接受的范围 …
An efficient high-throughput screening assay for rapid directed ...
2015年3月25日 · Here, a novel high-throughput screening (HTS) methodology for directed evolution of HHDH was developed based on the colorimetric determination of azide. In this …
Molecular modification of a halohydrin dehalogenase for kinetic ...
2019年3月1日 · Asymmetric synthesis of chiral epichlorohydrin (ECH) from 1,3-dichloro-2-propanol (1,3-DCP) using halohydrin dehalogenases (HHDHs) is of great value due to the …
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