Hi-T4™ 耐热 DNA 连接酶是 T4 DNA 连接酶的耐热突变体，可催化双链 DNA 或 RNA 中相邻的 5´ 磷酸末端和 3´ 羟基末端之间形成磷酸二酯键，比野生型 T4 DNA 连接酶更耐高热条件。 Hi-T4 耐热 DNA 连接酶可在高达 50℃ 的条件下连接平末端和粘性末端，并修复双链 DNA、RNA 或 DNA/RNA 杂交体中的单链切刻。 图 1：Hi-T4 耐热 DNA 连接酶的耐热性优于 T4 DNA 连接酶。 400 单位 T4 DNA 连接酶（NEB #M0202）或 Hi-T4 耐热 DNA 连接酶（NEB #M2622）在无 …

A thermotolerant variant of T4 DNA Ligase, Hi-T4™ DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini in duplex DNA or RNA and is designed to function at higher temperatures than wild type T4 DNA Ligase.

Hi-T4™ 耐热 DNA 连接酶是 T4 DNA 连接酶的耐热变体，该酶催化双链 DNA 或 RNA 上相邻的 5´ - 磷酸末端和 3´ - 羟基末端形成磷酸二酯键，经过改造其作用温度高于野生型 T4 DNA 连接酶。 Hi-T4 耐热 DNA 连接酶可在温度高达 50ºC 时进行平末端和粘性末端的连接以及修复双链 DNA 、RNA 或 DNA / RNA 杂交链的单链切刻。 浓度：400,000 cohesive end units/ml;缓冲液：T4 DNA Ligase Reaction Buffer，StickTogether™ DNA Ligase Buffer;反应条件：25℃;灭活条件：65℃。

A thermotolerant variant of T4 DNA Ligase, Hi-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher temperatures than wild type T4 DNA Ligase.

hi-t4™ 耐热 dna 连接酶是 t4 dna 连接酶的耐热变体，该酶催化双链 dna 或 rna 上相邻的 5´ – 磷酸末端和 3´ – 羟基末端形成磷酸二酯键，经过改造其作用温度高于野生型 t4 dna 连接酶。

Characteristics of Hi-T4 DNA Ligase: Hi-T4 DNA Ligase is an engineered variant of T4 DNA Ligase with improved thermostability compared to T4 DNA Ligase; Capable of joining blunt end and cohesive end termini, as well as repairing single stranded nicks in duplex DNA and some DNA/RNA hybrids

A thermotolerant variant of T4 DNA Ligase, Hi-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher temperatures than wild type T4 DNA Ligase.

A thermotolerant variant of T4 DNA Ligase, Hi-T4™ DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini in duplex DNA or RNA and is designed to function at higher temperatures than wild type T4 DNA Ligase.

StickTogetherTM DNA Ligase Buffer (2X) Set up the following reaction in a microcentrifuge tube on ice. (Hi-T4 DNA Ligase should be added last. Note that the table shows a ligation using a molar ratio of 1:3 vector to insert for the indicated DNA sizes.) Use NEBiocalculator to …

Hi-T4™ 耐热 DNA 连接酶是 T4 DNA 连接酶的耐热突变体，可催化双链 DNA 或 RNA 中相邻的 5´ 磷酸末端和 3´ 羟基末端之间形成磷酸二酯键，比野生型 T4 DNA 连接酶更耐高热条件。 Hi-T4 耐热 DNA 连接酶可在高达 50℃ 的条件下连接平末端和粘性末端，并修复双链 DNA、RNA 或 DNA/RNA 杂交体中的单链切刻。 图 1：Hi-T4 耐热 DNA 连接酶的耐热性优于 T4 DNA 连接酶。 400 单位 T4 DNA 连接酶（NEB #M0202）或 Hi-T4 耐热 DNA 连接酶（NEB #M2622）在无 …