2017年1月1日 · Hydrophobic interaction chromatography (HIC) is a high-resolution technique widely used for analysis and purification of biomolecules based on differences in their hydrophobicity. In this chapter, the basic principles of HIC and theories for the retention mechanisms are addressed, as well as the main parameters to consider for the optimization ...

2018年1月1日 · Hydrophobic interaction chromatography (HIC) is a widely used chromatographic technique that finds application at different stages of downstream processing. It was first described by Shepard and Tiselius in 1949 using the term “salting out chromatography.” The name HIC was later coined by Hjerten (1973), who was active in developing the technique.

2024年1月1日 · In this chapter, we aim to provide a practical guide for how we can approach essential analyses of Hi-C data to generate high-quality and publishable results. We also share our experience interpreting Hi-C results in the published …

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

疏水相互作用色谱 (HIC) 是一种蛋白质分离、纯化和表征的传统技术。 随着抗体偶联药物 (ADC) 的不断发展，HIC 在ADC 表征和分析上应用越来越广泛。 与其他技术不同，HIC 允许蛋白质在保持天然结构和活性的温和非变性条件下进行分析。 这一点非常重要，为HIC后续的研究提供了条件。 接下来，我们以一个传统的高效液相仪配合使用硫酸铵缓冲液为例，为大家阐述HIC的关键参数。 并对方法优化注意事项进行了总结。 简介. HIC的方法最早由 Tiselius 提出，他称此技术为" …

15 HiC. 15.1 Introduction to Chromatin Interaction and Organization; 15.2 Methods to Investigate 3D Genome Organization; 15.3 Topologically Associating Domains; 15.4 TAD Function and Loop Anchors; 15.5 Chromatin Compartments; 15.6 Computational Methods to Call Chromatin Loops; 15.7 Variations of Chromatin Interaction Technologies; 15.8 ...

ST. JOAQUIN CLASS BATCH 1993-1994 ( HIC CHAPTER ) - Facebook

In this chapter, we provide a description of the basic theory of HIC and how it is used to purify proteins in aqueous-based solutions. Following the theoretical background, the latest in HIC adsorbent technology is described, including a list of commonly used and …

TLOD, Inc. - Humble Intercontinental Chapter. 128 likes · 1 talking about this. Welcome to the Humble Intercontinental Chapter (HIC) of Top Ladies of Distinction, Incorporate (TLOD). TLOD is a...

2009年1月1日 · Hydrophobic interaction chromatography (HIC) is used in both analytical and preparatory scale protein purification applications. HIC exploits hydrophobic regions present in macromolecules that bind to hydrophobic ligands on chromatography adsorbents.