Similar to the classic 3C technique, Hi-C measures the frequency (as an average over a cell population) at which two DNA fragments physically associate in 3D space, linking …

We describe a method, Hi-C, to comprehensively detect chromatin interactions in the mammalian nucleus. This method is based on Chromosome Conformation Capture, in which chromatin is …

2021年1月30日 · In this study, we developed a novel HIC method capable of separating three mAbs with similar size, structure and charge properties, which enabled quantitation of their …

2023年2月13日 · Hydrophobic interaction chromatography (HIC) is a column chromatographic separation method that is commonly used to purify and isolate macromolecules such as …

2020年2月5日 · HIC is a technique that resolves drug-load variants of ADCs under native conditions based on their order of hydrophobicity. The method is widely employed for inter …

HIC forms one of the orthogonal techniques in a classic approach to purifying biomolecules by charge, hydrophobicity and size in a 3-step protocol. It complements adjacent purification steps

Hydrophobic interaction chromatography (HIC) is a technique for separation of proteins, peptides, and other biomolecules based on their relative degree of hydrophobicity. However, unlike …

HIC is typically a chromatographic separation technique and separates molecules according to differences in their surface hydrophobicity. The method is based on the interactions between …

2020年2月5日 · Hydrophobic interaction chromatography (HIC) is the state-of-the-art analytical tool to monitor conjugation-related critical quality attributes (CQAs) e.g. drug-load distribution …

Herein, we describe a generic HIC protocol for the screening, analysis, and characterization of ADCs using an ammonium sulfate buffer and a high-pressure liquid chromatography system. …