2014年4月1日 · HIEC cells are normal non-immortalized human intestinal epithelial crypt cells. Short term activation of the WNT pathway induces expression of stem cell markers. Co-inhibition of BMPs is required to trigger a robust stem cell signature. This provides a new model to study many aspects of intestinal stem cell biology.

2021年6月3日 · We developed a reproducible two-step protocol to induce human pluripotent stem cells to differentiate into highly expandable hIEC progenitors and a functional hIEC monolayer exhibiting intestinal molecular features, cell type diversity, and high activities of intestinal transporters and metabolic enzymes such as cytochrome P450 3A4 (CYP3A4).

In the HIEC cells of human embryonic origin, the missing TEER development across the cell membrane and the notorious holes observed via immunofluorescence suggest an immature epithelial cell line. This is in line with the impaired gut barrier function observed during early life in many mammalian species, which allows for passive transmission of ...

2014年4月1日 · In our laboratory, we isolated the first normal human intestinal epithelial crypt (HIEC) cell model from the human fetal intestine and in this study we investigated the expression of a panel of intestinal stem cell markers in HIEC cells under normal culture parameters as well as under conditions that mimic the stem cell microenvironment.

2024年3月10日 · Fraxin treatment exerts radioprotective effects by activating interleukin (IL)–22 in HIEC-6 cells. (A) HIEC-6 cells were pretreated with 160 μM fraxin and 0.1 μg/mL IL-22 neutralizing antibody 1 h before 6 Gy IR.

Results are reported as the mean (SEM) of triplicate samples. Significance was established at 95%, and determined by Student’s t test for non-paired values, and the Mann-Whitney U test for non-parametric values. RESULTS Effect of LPS on IEC growth. LPS proved to be a potent inhibitor of HIEC growth in a dose dependent manner.

2021年6月2日 · We developed a reproducible two-step protocol to induce human pluripotent stem cells to differentiate into highly expandable hIEC progenitors and a functional hIEC monolayer exhibiting intestinal molecular features, cell type diversity, and high activities of intestinal transporters and metabolic enzymes such as cytochrome P450 3A4 (CYP3A4).

需要先进的技术来生成具有细胞多样性和功能的人类肠上皮细胞（hIEC），以预测人类口服药物的吸收并研究正常的肠上皮生理学。 我们开发了一种可重复的两步方案，诱导人类多能干细胞分化为高度可扩展的 hIEC 祖细胞和功能性 hIEC 单层，表现出肠道分子特征、细胞类型多样性以及肠道转运蛋白和代谢酶（如细胞色素 P450 3A4）的高活性。 CYP3A4)。 功能性 hIEC 比 Caco-2 细胞更适合预测 CYP3A4 代谢并在肠道吸收的化合物。 该系统是在不影响细胞多样性和功能的情 …

2000年1月1日 · The recent availability of spontaneously proliferating, non-transformed human crypt intestinal epithelial cells (HIEC) affords an opportunity to investigate lipid metabolism in undifferentiated enterocytes.

Compared to immature hIECs, functional hIECs formed a structurally polarized monolayer, as evident from the scanning electron microscopy (SEM) images and the polarized distribution of apical...