Ligation of the oligonucleotide will generate a HindIII site at the 3′-end of cDNA because of the presence of the poly(A) tail (Dorssers & Postmes, 1987). Inactivate the ligase by heating at …

Thus HindII and HindIII are two enzymes from Haemophilus influenzae strain Rd. Restriction endonuclease and modification methyltransferases (ENases and MTases) are sometimes …

2012年11月1日 · The HindIII enzyme recognizes the sequence: 5′-AAGCTT-3′ and cleaves the DNA, leaving a 5′ overhang of: 5′-AGCT-3′. This cleavage provides a template for labeling the …

1977年9月1日 · Fragment F of a HindIII digest of X is only 0.1% X unit larger than HindII+III fragment B4. When the HindIII- unique fragment F is eluted from a preparative-scale gel and …

2020年8月1日 · The HindIII variant exists 495 bp from the intron-8 towards the splice site and effect the RNA splicing. The H-allele in this variant may enhance the enzyme activity ( Xie, …

1980年1月1日 · HindlII is reported to have an altered specific- ity in the presence of MnZ+. e Ionic Requirements. Ionic and pH requirements have been evaluated semiquantitatively by gel …

2008年9月1日 · Binding of HindIII wild type (lane 1) and mutant type (lane 2) with purified human TBP. Lanes 1 and 2 contain identical amounts of HindIII oligos and TBP, but the affinity of TBP …

1977年9月1日 · A cDNA library has been constructed from an RNA preparation highly enriched in silkmoth chorion mRNAs. Many distinct clones have been identified from this library using a …

1977年9月1日 · The production and use of the 6 kb cosmid pHC79, a derivative of pBR322, is described. It can be used for cloning of fragments cleaved by EcoRI, ClaI, BamHI (also BglII, …

2017年7月1日 · We previously described the use of HindIII, which has an average fragment length of ∼4 kb. Previous high resolution Hi-C libraries have used MboI or DpnII [13] , [14] to …