2003年1月31日 · To gain insight into the mechanisms by which HP1 mediates formation and maintenance of heterochromatin in living cells, we investigated the kinetic binding of green fluorescent protein–tagged HP1 (GFP-HP1) to native chromatin using in vivo microscopy.

2015年8月4日 · Experiments based on fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRAP) have revealed the HP1 proteins to adopt different dynamics in regions of...

2009年12月2日 · With FRAP, we measured an 11% HP1α fraction immobilized for at least 60 s in heterochromatin, which can be assigned to the stably bound HP1α fraction detected in the FLIP experiments. FCS measurements of HP1 provide spatially resolved effective diffusion coefficients, anomalous diffusion parameters, and concentrations

Using fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRAP) we found that in interphase cells most HP1 molecules (50–80%) are highly mobile (recovery halftime: t 1/2 ≈ 0.9 s; diffusion coefficient: D ≈ 0.6–0.7 μm 2 s-1).

2014年4月25日 · Numerous FRAP studies, in conjunction with kinetic modelling, have shown that at steady state equilibrium the nuclear HP1 pool can be separated into three kinetic fractions: a highly mobile ...

2008年4月27日 · DNA breaks swiftly mobilize heterochromatin protein 1 (HP1)-β (also called CBX1), a chromatin factor bound to histone H3 methylated on lysine 9 (H3K9me). Local changes in histone-tail...

Here we describe how to perform FRAP and FLIP assays of chromatin proteins, including H1 and HP1, in mouse embryonic stem (ES) cells. In a FRAP experiment, cells are transfected, either transiently or stably, with a protein of interest fused with the green fluorescent protein (GFP) or derivatives thereof (YFP, CFP, Cherry, etc.).

2021年3月4日 · We propose that multivalent interactions of HP1β with H3K9me3-modified nucleosomes via its chromodomain and dimerization via its chromoshadow domain enable phase separation and contribute to the formation of heterochromatin compartments in vivo.

2017年11月21日 · A key factor in heterochromatin structure is heterochromatin protein 1 (HP1), which was originally described in Drosophila as a suppressor of position-effect variegation (Eissenberg et al., 1990). Mammals harbor three HP1 isotypes termed HP1α, HP1β, and HP1γ (Jones et al., 2000).

2009年6月1日 · FRAP experiments reveal that in contrast to HP1, SUV420H2 is strongly associated to pericentric heterochromatin. Then, the fraction of SUV420H2 captured and characterized by TAP/MS is a soluble fraction which may be in a stable association with HP1.