Human Heterochromatin Protein 1 Isoforms HP1
We show here that in human cells the overexpression of green fluorescent protein-tagged heterochromatin protein 1 (GFP-HP1) or nontagged HP1 isoforms HP1 Hsα or HP1 Hsβ, but …
GFP-HP1alpha - Addgene
Plasmid GFP-HP1alpha from Dr. Tom Misteli's lab contains the insert HP1 alpha and is published in Science. 2003 Jan 31. 299 (5607):721-5. This plasmid is available through Addgene.
Nanobody-mediated control of gene expression and epigenetic memory - Nature
2021年1月22日 · We show that an antiGFP nanobody can be used to simultaneously visualize GFP-tagged chromatin regulators and control gene expression, and that nanobodies against …
Regulation of HP1–chromatin binding by histone H3 ... - Nature
2005年10月12日 · Tri-methylation of histone H3 lysine 9 is important for recruiting heterochromatin protein 1 (HP1) to discrete regions of the genome, thereby regulating gene …
Mouse Heterochromatin Adopts Digital Compaction States …
2020年4月16日 · HP1 can bridge chromatin segments and form liquid droplets, but the biophysical principles underlying heterochromatin compartmentalization in the cell nucleus are …
GDV1 induces sexual commitment of malaria parasites by antagonizing HP1 ...
2018年3月16日 · In this study, we identified Plasmodium falciparum gametocyte development 1 (GDV1) as an upstream activator of sexual commitment. We found that GDV1 targeted …
Uncoupling the distinct functions of HP1 proteins during ...
2023年11月28日 · Our results demonstrate the role of HP1 proteins in regulating histone modification crosstalk during establishment and identify a genetically separable function in …
Compartmentalization of HP1 Proteins in Pluripotency Acquisition …
2018年2月2日 · In this article, Sridharan and colleagues perform proteomic characterization of the heterochromatin protein 1 (HP1) family in reprogramming and pluripotency. Depletion of HP1γ …
HP1 proteins regulate nucleolar structure and function by …
2022年12月19日 · Hp1α/β deficiency reduces cell proliferation, ribosomal RNA biosynthesis and mobility of Nucleophosmin, a major nucleolar component. Nucleolar integrity and function …
Phase separation drives heterochromatin domain formation
2017年6月21日 · We show that Drosophila HP1a protein undergoes liquid–liquid demixing in vitro, and nucleates into foci that display liquid properties during the first stages of heterochromatin …
HP1 Promotes the Centromeric Localization of ATRX and Protects …
Our research demonstrated that HP1α, HP1β, and HP1γ facilitate the positioning of ATRX within the mitotic centromere area through their interaction with the first two [P/L]-X-V-X- [M/L/V] …
Heterochromatin Protein 1β (HP1β) has distinct functions and …
2015年9月28日 · Here we identify Heterochromatin Protein 1β (HP1β) as an essential protein for proper differentiation, and, unexpectedly, for the maintenance of pluripotency in ESCs. In …
Plasticity of HP1 proteins in mammalian cells - PubMed
2007年10月1日 · We have compared the distribution of endogenous heterochromatin protein 1 (HP1) proteins (alpha, beta and gamma) in different epithelial lines, pluripotent stem cells and …
Heterochromatin protein 1 alpha (HP1α) undergoes a monomer to …
2024年8月28日 · Specifically, we find that HP1α monomers, which are the dominant species according to fluorescence fluctuation spectroscopy (FFS) of HP1α tagged to eGFP, impart a …
Heterochromatin Dynamics in Mouse Cells - ScienceDirect
1999年10月1日 · We demonstrate that chromatin assembly factor 1 (CAF-1) binds to mouse HP1 proteins via an N-terminal domain of its p150 subunit, a domain dispensable for nucleosome …
Drosophila STAT is required for directly maintaining HP1 ... - Nature
2008年3月16日 · To investigate how STAT92E activation by phosphorylation leads to heterochromatin destabilization, we examined STAT92E–GFP and HP1 localization in ex vivo …
LHP1 Interacts with ATRX through Plant-Specific Domains at Specific ...
2018年8月6日 · Anti-GFP antibody was used to detect LHP1::GFP. (B) Identification of ATRX regions that directly interact with LHP1 using the yeast two-hybrid assay. Various truncated …
Plant HP1 protein ADCP1 links multivalent H3K9 methylation
2018年11月13日 · Here, we report on the discovery of ADCP1, a plant-specific triple tandem Agenet protein, as a multivalent H3K9me reader in Arabidopsis, and establish that ADCP1 is …
Functions of HP1 proteins in transcriptional regulation
2022年5月7日 · HP1 proteins are highly conserved, and many eukaryotic genomes contain multiple HP1 genes. Given the presence of multiple HP1 family members within a genome, …
Mammalian HP1 Isoforms Have Specific Roles in ... - ScienceDirect
2017年11月21日 · Analysis of Hp1α −/−, Hp1β −/−, and Hp1γ −/− MEFs show that HP1 proteins have both redundant and unique functions within pericentric heterochromatin (PCH) and also …