2021年4月14日 · Our work identifies HP1a as an epigenetic regulator that is involved in establishing the global structure of the genome in the early embryo. In metazoans, fertilization triggers global de novo...

2024年8月28日 · Selective siRNA knockdown (KD) of endogenous HP1α in wild type HeLa (HeLa KD) and the cell lines exogenously transfected with eGFP-HP1α, eGFP-HP1α I165E or HP1α I165E (HeLa eGFP-HP1α+KD, HeLa eGFP-HP1αI165E+KD, and HeLa HP1αI165E+KD) was achieved via transfection of three siRNA duplexes designed to target the mRNA 3′ untranslated ...

2024年12月3日 · HP1 binding to RNA is shown to facilitate its localization at and assembly of heterochromatin in metazoans. Likewise, recruitment of Swi6/HP1 to centromere depends on the RNAi pathway in fission yeast; paradoxically, Swi6/HP1 is also thought to play a role in RNA turnover. Here we provide evidence in support of RNAi-independent recruitment of Swi6.

The HP1-rescue construct reverses the structural effects of HP1-KD (reduced contact frequency between the pericentromeric regions, as well as inter-chromosome arm interactions and compaction defects).

2022年2月15日 · Heterochromatin protein 1 (HP1) governs heterochromatin organization through maintaining protein stability and chromatin binding of H3K9 methyltransferases and demethylases in mESCs. HP1 interaction‐defective H3K9 MTs undergo protein degradation. HP1 depletion reduces H3K9 MTs and H3K9 DMs protein levels.

2019年10月16日 · The S. pombe HP1 protein Swi6 couples chromatin compaction to phase separation by dynamically exposing buried histone residues within nucleosomes. Heterochromatin affects genome function at many...

2021年4月3日 · NCBI's Gene Expression Omnibus (GEO) is a public archive and resource for gene expression data.

2018年10月10日 · Heterochromatin protein 1 γ (HP1γ) is a well-known chromatin protein, which regulates gene silencing during the execution of processes associated with embryogenesis, organ...

Based on cytological and repeat specific analysis, we conclude that depletion of HP1 results in a global advance of centromeric heterochromatin ... ... determine if replication timing changes...

2008年12月26日 · HP1 proteins are a highly conserved family of eukaryotic proteins that bind to methylated histone H3 lysine 9 (H3K9) and are required for heterochromatic gene silencing. In fission yeast, two HP1 homologs, Swi6 and Chp2, function in heterochromatic gene silencing, but their relative contribution to silencing remains unknown.