HpaII is an isoschizomer of MspI. Inhibited by salt concentrations > 50 mM KCl. Blocked by CpG methylation. Why Choose Recombinant Enzymes? Is HpaII inhibited by salt? Is HpaII affected …

HpaII (IntEnz: EC 3.1.21.4) is a restriction enzyme obtained from the microorganism Haemophilus parainfluenzae. It is a DNA restriction enzyme, therefore it has the ability to cut the DNA from …

Thermo Scientific HpaII restriction enzyme recognizes C^CGG sites and cuts best at 37C in Tango buffer. Thermo Scientific conventional restriction endonucleases are a large collection …

HpaII Methyltransferase recognizes the same sequence as the MspI Methyltransferase, but modifies the internal cytosine residue (C 5) of the sequence CCGG. An E. coli strain that …

5-methylcytosine is a prominent epigenetic DNA modification which plays an important role in up- and down-regulation of gene expression. The Thermo ScientificTM EpiJETTM DNA …

2021年1月15日 · The MSREs HpaII, Hin6I, AciI, and HpyCH4IV together cover six of the nine canonical CpG methylation sites of the FOXP3 TSDR . A demethylated cytosine located at any …

1980年1月1日 · This chapter discusses the preparation and properties of the HpaI and HpaII endonucleases. Two restriction endonuclease activities, HpaI and HpaII are isolated from …

The gene for the HpaII methylase has been cloned into E. coli and its nucleotide sequence has been determined. The DNA of the clones is fully protected against cleavage by the HpaII …

HpaII-amplifiable fragments are defined as those that can be (a) amplified using our LM-PCR approach and (b) into which we can place an oligonucleotide for microarray interrogation, …

The secondary digestion of these fragments with methylation-sensitive enzymes, BstUI or HpaII, helps remove umethylated fragments. The methylated products are first amplified by primers …