在RNAi研究中，通常通过向细胞内导入发夹RNA（hairpin RNA， hpRNA）来引起干扰，即在构建植物转基因的双元载体时，通过强启动子驱动一个“正向序列一环一互补序列”的转录单元进行转录，转录出的RNA可形成hpRNA， hpRNA通过DICER酶的作用，被加工成小干扰RNA （small ...

2022年7月7日 · Hairpin RNA (hpRNA) transgenes are the most successful RNA interference (RNAi) method in plants. Here, we show that hpRNA transgenes are invariably methylated in the inverted-repeat (IR)...

2016年4月26日 · 摘要：RNAi技术在基因功能鉴定和功能基因表达调控中发挥着重要作用。 hpRNA干扰载体被广泛用于植物的RNAi研究。 对植物hpRNA干扰载体的构建方法进行综述，并分析其优缺点，为植物hpRNA干扰载体构建方法的选择提供参考。 constructionofplanthpRNAvector. 本试验步骤和必要方法。 在RNAi研究中，通常通. 来表达双链RNA，以沉默目的基因的表达。 在植物. 因此，ihpRNA. 表达载体被广泛用于植物的基因沉默研究。 为了方. 下5类。 …

2024年7月7日 · Pathogenic allele silencing is a promising treatment for genetic hereditary diseases. Here, we develop an RNA-cleaving tool, TaqTth-hpRNA, consisting of a small, chimeric TaqTth, and a hairpin RNA guiding probe. With a minimal flanking sequence-motif requirement, in vitro and in vivo studies show TaqTth-hpRNA cleaves RNA efficiently and ...

3p-hpRNA (tlrl-hprna) is a specific RIG-I agonist generated by in vitro transcription of an influenza A virus sequence. This triphosphate dsRNA (3pRNA) is endotoxin-free and its potency is validated on a RIG-I reporter cell-based assays.

2019年6月15日 · Hairpin-structured (hp) RNA has been widely used to induce RNA interference (RNAi) in plants and animals, and an in vivo expression system for hpRNA is important for large-scale RNAi applications. Bacterial expression systems have so far been developed for in vivo expression of hpRNA or double-stran …

RNA silencing is an evolutionarily conserved mechanism in eukaryotes. It is induced by double-stranded RNA (dsRNA) or hairpin structured RNA (hpRNA), involving common factors including Dicer or Dicer-like (DCL) and Argonaute (AGO) family proteins [1 - 3].

2016年11月29日 · Understanding the functions encoded by plant genes can be facilitated by reducing transcript levels by hairpin RNA (hpRNA) mediated silencing. A bottleneck to this technology occurs when a gene...

2022年7月7日 · Hairpin RNA (hpRNA) transgenes are the most successful RNA interference (RNAi) method in plants. Here, we show that hpRNA transgenes are invariably methylated in the inverted-repeat (IR) DNA and the adjacent promoter, causing transcriptional self-silencing.

2024年5月13日 · 发夹 RNA 载体（hpRNA ) 的产生 构建 hpRNA 的方法有多种。 可以利用标准的植物转化载体进行构建，这种方法需要将每个目的基因对应的发夹编码结构都重新构建到载体上。