2007年6月19日 · We monitored in vivo the fate of the paternal chromatin upon double fertilization in crosses between wild-type ovules and pollen with sperm cells marked by H3.3-mRFP1 (Figure 2) and HTR12-GFP (Figure 3).

2014年11月17日 · To visualize mitotic defects during microgametogenesis in mos7-5, the ProHTR12:HTR12:GFP transgene, a centromere marker (15), was crossed into a MOS7/mos7-5 heterozygote. Consistent with Chen et al. (16), in microspores from wild-type plants, HTR12:GFP was observed at all five chromosomal centromeres in the haploid microspore (Fig. 3 A).

Immuno-localization studies and live-imaging analyses have shown that HTR12 accumulates at heterochromatin around centromeres [13, 16, 17]. During male gameto-genesis, HTR12-GFP was first expressed in the nucleus of unicellular haploid …

2005年9月29日 · Centromeres were in vivo tagged by expressing a fusion of centromere-specific histone 3 (HTR12) with GFP variants. By in situ imaging of the transgenic plants, we addressed how the centromeres are organized in the interphase nucleus in three dimensions, whether this organization is different in endoreduplicated cells, and whether centromeres ...

2010年3月25日 · Centromeres are the chromosomal loci that attach to spindle microtubules to mediate faithful inheritance of the genome during cell division. They are epigenetically specified by incorporation of...

HTR12 is the Arabidopsis homolog of the centromere-specific variant CENH3 ( Talbert et al., 2002). When we examined a C-terminal HTR12-GFP fusion (Fang and Spector, 2005), we observed a ...

2010年1月19日 · Three different distribution patterns (centromeric: Cen, dispersed: Disp, and centromeric+dispersed: Cen+Disp) were observed in Arabidopsis cells transformed with HTR12-GFP. As expected, most cells showed a Cen pattern, which clearly indicates centromeric co-localization of immunosignals from GFP and AtMIS12 (Fig. 2e–h).

Encodes a centromere-identifying protein histone H3 variant. Localized at centromeres in both mitotic and meiotic cells. Aurora3 phosphorylates CENH3 at S65; this post-translational modification is required for the proper development of the floral meristem.

2007年6月19日 · We overcame technical limitations to live imaging of the fertilization process in Arabidopsis thaliana and studied dynamics of the male-gamete-specific H3.3 [12] and the centromeric Histone Three Related 12 (HTR12) [13]. The double-fertilization process in plants produces the zygote and the embryo-nourishing endosperm [14].

2019年6月11日 · To elucidate the effect of genome doubling on chromosome arrangement in living Arabidopsis plants, we transformed Col-0 and 4 × Col-0 Arabidopsis with HTR12-GFP, a centromere-specific histone H3 marker, using flower dipping (52, 53).