2009年5月28日 · HTRF (Homogeneous Time Resolved Fluorescence) is the most frequently used generic assay technology to measure analytes in a homogenous format, which is the ideal platform used for drug target studies in high-throughput screening (HTS). This technology combines fluorescence resonance energy transfer …

Overall, HTRF is a highly sensitive, robust technology for the detection of molecular interactions in vitro and is widely used for primary and secondary screening phases of drug development. This review addresses the general principles of HTRF and its current applications in drug discovery.

HTRF™ (Homogeneous Time Resolved Fluorescence) is a no-wash technology that combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. This technology can be utilized in cellular or biochemical assays, employing competitive and non-competitive formats.

2020年6月16日 · Homogeneous Time-Resolved Fluorescence (HTRF ®) is a Time-Resolved FRET (TR-FRET) immunoassay developed by Cisbio. This technology combines Time-Resolved Fluorescence (TRF) detection with Fluorescence Resonance Energy Transfer (FRET) and is mainly used for drug screening in high-throughput.

HTRF® is the premier TR-FRET (Time-resolved Fluorescence Resonance Energy Transfer) technology on the market. It brings together the sensitivity of fluorescence with the homogenous nature of FRET (Fluorescence Resonance Energy Transfer) and the …

Time-Resolved Fluorescence (TRF) and TR-FRET (HTRF) are the powerful tools for drug discovery which enable the analysis of molecular interactions in biochemical processes like kinase assays, cellular signaling pathways, protein-protein interactions.

Streptavidin has been labeled with d2. Biotin binds to Streptavidin with high affinity (Ka=1015M-1). The binding is rapid and stable, making it an ideal choice for use in a variety of assays such as enzyme assays, protein-protein binding assays and molecular biology assays. Harness avidity without compromising on pharmacology.

2009年5月28日 · HTRF (Homogeneous Time Resolved Fluorescence) is the most frequently used generic assay technology to measure analytes in a homogenous format, which is the...

HTRF®, is the premier TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) technology on the market. TR-FRET technologies such as HTRF bring together the sensitivity of fluorescence with the homogeneous nature of FRET (Fluorescence Resonance Energy Transfer) and the low background of time resolution.

2024年12月20日 · HTRF (Homogeneous Time-Resolved Fluorescence)技术源自于诺贝尔奖成果，是一种同时兼具TR (Time Resolved，时间分辨)技术和FRET (Fluorescence Resonance Energy Transfer，荧光能量共振转移)技术的在均相（溶液）反应体系中的分析技术，经过三十余年的技术沉淀，已被超万篇高分文献引用。 TR技术利用稀土元素中镧系元素 (Eu、Tb、Sm等)的独特性质，其荧光比普通荧光持续时间更长，普通荧光的半衰期为纳秒级，镧系元素的半衰期是毫秒 …