qDSB-Seq is a general method for genome-wide quantification of …
2019年5月24日 · Here we propose quantitative DSB sequencing (qDSB-Seq), a method providing both DSB frequencies per cell and their precise genomic coordinates. We induce spike-in …
i-BLESS is an ultra-sensitive method for detection of DNA
2018年10月31日 · DNA double-strand breaks (DSBs) are one of the most lethal types of DNA lesions 1, being a primary source of chromosome translocations and deletions 2. Since DSBs...
Hydroxyurea-Stalled Replication Forks Become Progressively Inactivated ...
Hydroxyurea (HU) depletes the cells of dNTPs, which initially results in stalled replication forks that, after prolonged treatment, collapse into DSBs. Here, we report that stalled replication …
Hu, Jiazhi - dash.harvard.edu
Here we describe a linear amplification–mediated modification of a previously published high-throughput, genome-wide translocation sequencing (HTGTS) method that robustly detects …
In vitro reconstitution of meiotic DNA double-strand-break …
2025年2月19日 · Chromosome spreading with immunostaining for antibodies against RPA2 (a marker for DSB repair), phospho-histone H2AX (γH2AX, a marker for the DNA damage …
DNA replication stress triggers rapid DNA replication fork ... - PLOS
2018年7月30日 · We treated the human colon carcinoma cell line RKO with 0.5, 2 and 8 mM hydroxyurea (HU), an inhibitor of dNTP biogenesis, for 1–24 hours. This DRS induced DSBs …
Nature子刊：中山大学松阳洲团队发现DNA损伤修复通路的新选 …
2023年6月19日 · DNA双链断裂（DNA double-strand break，DSB）是一种常见且高度有害的DNA损伤形式，严重威胁着基因组稳定性和细胞存活。 DSB修复缺陷或失调与肿瘤、衰老以 …
Dual Roles of Poly(dA:dT) Tracts in Replication Initiation and Fork ...
The DSB site is defined as edge of sharp peak flanking the gap between sharp and broad ends of the DSBs. Defining HU-DSB zones. The END-seq DSB sites within 20 kb of each other were …
丙戊酸通过抑制 RPA2 过度磷酸化介导的 DNA 修复途径使乳腺癌 …
采用多种乳腺癌细胞模型来研究 0.5 mm vpa 和 2 mm hu 的安全浓度是否会导致 dna 双链断裂 (dsb) 并影响细胞存活。 此外，通过细胞生物学检测探索了潜在的机制，包括克隆存活、同源重 …
EMBO Journal | 华中农业大学严顺平团队在植物DNA损伤应答领域 …
生物主要通过两种途径修复dsb，即非同源末端连接（nhej）和同源重组（hr）。nhej 修复途径是一个相对快速但容易出错的过程，而hr修复途径是一种相对缓慢但精准的修复途径。 dsb修复 …