2021年10月1日 · We cultured the well-characterised SH-SY5Y human neural cell line to produce high purity cultures of cells differentiated to express a neuronal phenotype, and designed a protocol to maintain these cells in culture until they accumulated biomarkers of cellular ageing.

SH-sy5y是神经细胞瘤细胞，从来源上讲是人源的，但是这株细胞严格上属于瘤源。 而PC12是鼠源的。 我们的实验想从基因水平对比，诱导出的细胞与人源神经元的相似性，所以求助各位有没有好的解决方案或者替代实验细胞！

The SH-SY5Y cell line is a thrice cloned subline of the neuroblastoma cell line SK-N-SH (ATCC HTB-11), which was established in 1970 from a metastatic bone tumor from a 4-year-old cancer patient. Applications include use as a transfection host or in immunology, neuroscience, and toxicology research.

2023年7月1日 · We have used the human SH-SY5Y neuroblastoma cell model to evaluate a panel of 93 mRNA markers that are frequent in Neuronal diseases and functional annotations and also differentially expressed during retinoic acid-induced differentiation in the cell model.

2024年1月12日 · For this purpose, we generated a three-dimensional (3D) neuronal culture, by employing the readily available human neuroblastoma SH-SY5Y cell line, and a new differentiation protocol.

2021年7月1日 · We used the human neuroblastoma SH-SY5Y cells line to study responses to PFOA exposure, as it is a widely used cell model in neurodegenerative studies (Xicoy et al., 2017) and there is evidence that PFAS alters catecholamine pathways in the brain (Salgado et al., 2016). We hypothesized that exposure to PFOA would negatively affect mitochondrial ...

2016年2月17日 · The following procedure describes an easy-to-follow, reproducible method to obtain homogenous and viable human neuronal cultures, by differentiating the chromosomally stable human neuroblastoma cell line, SH-SY5Y. This method integrates several previously described methods 1-4 and is based on sequential removal of serum from media. The timeline ...

2016年2月17日 · The following procedure describes an easy-to-follow, reproducible method to obtain homogenous and viable human neuronal cultures, by differentiating the chromosomally stable human neuroblastoma cell line, SH-SY5Y. This method integrates several previously described methods (1-4) and is based on sequential removal of serum from media.

2023年8月30日 · Here, we report that lytic HSV‐2 infection of human neuron‐like SH‐SY5Y cells and primary human and murine brain cells leads to cell death mediated by gasdermin E (GSDME).

In this context, human cell models appear relevant in elucidating cellular and molecular impacts of neurotoxicants and facilitating prioritization of in vivo testing. The SH-SY5Y human neuroblastoma cell line (ATCC® CRL-2266™) is one of the most used cell lines in neurosciences, either undifferentiated or differentiated into neuron-like cells.