Crystal structure of hydrogenase maturating endopeptidase HycI …
2009年11月13日 · In our crystal structure, three specific metal binding sites (Ca1–3) were identified and these metal ions are possibly involved in the C-terminal cleavage of HycE. …
Interaction of the Hydrogenase Accessory Protein HypC with HycE…
1998年2月11日 · Maturation of the large subunit of E. coli hydrogenase 3, HycE, requires the action of seven accessory proteins. The HycI protease catalyses a C-terminal proteolytic …
产氧克雷伯菌HP1的耐氧氢酶的表征和克隆。,Research in …
2010年12月29日 · 构建了hycE基因缺失突变体ΔhycE，用于阐明hyc-操纵子在氢代谢中的功能。 与野生型菌株HP1相比，该突变菌株在O（2）胁迫条件下在甲酸，丙酮酸钠和葡萄糖存在下 …
Dissection of the maturation reactions of the [NiFe] hydrogenase 3 …
2000年5月12日 · The steps in the maturation of the precursor of the large subunit (pre-HycE) of hydrogenase 3 from Escherichia coli taking place after incorporation of both iron and nickel …
使用转基因产<em>气肠杆菌</em>改善发酵产氢<em ... - X-MOL
产气肠杆菌的[NiFe]-氢化酶3包含一个60 kDa的大亚基（HycE）和一个30 k Da的小亚基（HycG）。 使用pGEX-4T-2-cat载体分别在产气大肠杆菌中过表达HycE基因和HycG基因， …
Protein interactions and localization of the Escherichia coli …
2011年12月16日 · Although the isolated proteins have been characterized, little is known about how they interact with each other and the hydrogenase 3 large subunit, HycE. In this study the …
Solution Structure and Backbone Dynamics of an Endopeptidase …
HycI is an endopeptidase of the M52 family and responsible for the C-terminal cleavage of the large subunit of hydrogenase 3 in Escherichia coli. Although extensive studies were …
Interaction of the hydrogenase accessory protein HypC with HycE…
1998年3月3日 · Maturation of the large subunit of E. coli hydrogenase 3, HycE, requires the action of seven accessory proteins. The HycI protease catalyses a C-terminal proteolytic …
Protein engineering of hydrogenase 3 to enhance hydrogen
2008年5月1日 · The large subunit (HycE, 569 amino acids) of Escherichia coli hydrogenase 3 produces hydrogen from formate via its Ni–Fe-binding site. In this paper, we engineered HycE …
Crystal structure of hydrogenase maturating endopeptidase HycI from ...
2009年11月1日 · Here, we present the crystal structure of HycI at 1.70 Å resolution. The crystal structure resembles the recently reported solution structure (NMR) of the same protein and the …