请问各位，Isolectin-B4 是特异性标记血管的么？血管的哪一个成分？染色是否就像DAPI一样不需要二抗？问题较为急切，非常希望大家能帮到我，谢谢！

2014年12月11日 · Use a sequential scan to detect the nuclei stained with DAPI using a 405-nm laser, for IB4 use a 561-nm laser and for EB use a 633-nm laser.

2019年7月5日 · Here we show, using genetic reporter and conditional deletion strategies, that myeloid cells regulate tip cell numbers and Dll4 expression via the Notch ligand Dll1 during vascular development in...

2021年5月25日 · Here we demonstrate that angiogenesis is started near Aß plaques in both AD mouse models and human AD samples. However, endothelial cells express the molecular signature of non-productive...

2024年12月2日 · 免疫荧光（Immunofluorescence，IF）技术是一种强大的生物学工具，它利用荧光标记的抗体来精确定位和定性分析组织或细胞内的特定抗原。 在细胞生物学研究中，细胞免疫荧光染色尤为常见，它能够帮助科学家们深入了解细胞内部的结构和功能。 接下来，我们将详细介绍细胞爬片免疫荧光实验的操作步骤及一些小技巧。 • 仪器和耗材：洁净工作台、CO2恒温培养箱、涡旋混合器、移液枪、正置荧光显微镜、成像系统。 • 试剂：4%多聚甲醛、破膜剂（如0.5% …

NF200 (blue, pseudocolored), IB4 (green) and CGRP (red) positive neurons and overlap of populations, in the form of double-positive cells, are visible. Nuclear counterstaining was performed using...

2014年2月13日 · We show in a mouse model that IB4 (+) neurons play an important role in cancer-induced mechanical allodynia, while TRPV1 mediates cancer-induced thermal hyperalgesia. Characterization of the sensory fiber subtypes responsible for cancer pain could lead to the development of targeted therapeutics.

We utilized confocal laser scanning microscopy (CLSM) to detect the presence of alpha-Gal epitopes (IB4) and cell nuclei (DAPI). Results: alpha-Gal specific IgG was significantly increased 3 months after implantation of bioprostheses compared to preoperative values (p < 0.001) and was significantly higher than alpha-Gal specific IgG levels of ...

2022年3月18日 · The following steps outline how to fluorescently label Z-depth reduced adipose tissues or tissue blocks with primary antibodies, secondary antibodies, isolectin-IB 4, and DAPI. It is important that, to reduce non-specific binding and …

2025年1月3日 · To begin, obtain Isolectin B4 or IB4 and DAPI-stained mouse lung tissue sections. Acquire images under a fluorescence microscope with a 10x objective. Import the images to ImageJ software and select them.