We show here that E2A DNA binding is lowered in primary immature thymocytes consequent to T cell receptor (TCR)-mediated ligation. Whereas expression of E2A mRNA and protein are unaltered,...

It is established that E2A and its antagonist, Id3, modulate developmental progression at the pre-TCR receptor (pre-TCR) and TCR checkpoints. Here we demonstrate that Id3 expression is elevated beyond the pre-TCR checkpoint, remains high in naive T cells and shows a bimodal pattern in the effector/memory population.

2016年3月1日 · The interaction mimics of the antagonist effect of ID3 with E2A/E12 provides a structural insight into how the helix–loop–helix domain might mediate the structural mechanism and vice versa. Accordingly, the structure of ID3 and E2A/E12 was predicted and analyzed for their stability through 50 ns MD simulation. Ensemble conformations of ID3 ...

2001年12月1日 · Id3 is essential for normal thymocyte positive selection. The effects of an absence of Id3 on positive selection can be suppressed by a deficiency of E2A, showing that E2A and Id3...

2024年2月7日 · ID3 directly represses transactivation of the inhibitory receptor Sirpa by inhibiting binding of the E-box E2A and the MAP-kinase target ELK1 to the promoter/enhancer regions of the Sirpa gene,...

2024年2月25日 · 作者通过深度学习分析数据，以及使用cut&run分析等实验手段，表明id3阻止了e2a和elk1在sirpa启动子以及上游和内含子sirpa增强子区域的结合。 此外，靶向E2A和ELK1的shRNA都将ID3缺陷型KCs中的SIRPA表达降低到野生型水平。

We show that down-regulation of Id3 in B cells is essential for releasing E2A and E2-2, which in a redundant manner are required for antigen-induced B cell differentiation. We demonstrate that this pathway controls the expression of multiple key factors, including Blimp1, Xbp1, and CXCR4, and is therefore critical for establishing the ...

The basic helix-loop-helix (bHLH) proteins E12 and E47 and an inhibitor HLH protein, Id3, play key roles in thymocyte differentiation. We show here that E2A DNA binding is lowered in primary immature thymocytes consequent to T cell receptor (TCR)-mediated ligation.

2018年1月24日 · In response to pre-TCR and TCR signals, inhibition of E protein activity by Id proteins plays a critical role in promoting the differentiation and positive selection of conventional αβ T cells, such that disruption of Id2 and Id3 impairs conventional αβ T cell development beyond the TCR checkpoint .

2015年12月4日 · Inhibitor of DNA binding protein 3 (ID3) has long been characterized as an oncogene that implicates its functional role through its Helix-Loop-Helix (HLH) domain upon protein-protein interaction. An insight into the dimerization brought by this domain helps in identifying the key residues that favor …