Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins based on their isoelectric point (pI). The pI is the pH at which a protein has no net charge and does not move …

Scheme of isoelectric focusing with immobilized pH gradient (IPG) gels. Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different charged molecules by …

Isoelectric focusing (IEF) gels separate proteins based on their net charge rather than their molecular weight. IEF gels are cast with carrier ampholytes to create a pH gradient within the …

Isoelectric focusing (IEF) is mostly run in horizontal gels because of several reasons: - As IEF separates only according to the charge, the gel matrix must contain large pore sizes. Such a …

Vertical IEF gels effectively create a pH gradient so proteins separate according to their unique pI. IEG gels can be used to readily detect minor changes in a protein due to deamination, …

等电聚焦（IEF）是基于蛋白等电点（pI）来分离蛋白的电泳技术。pI 是指蛋白不带净电荷且在电场中不运动时的 pH 值。Novex IEF 预制胶可有效建立 pH 梯度条件，因此蛋白可根据其独特 …

Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins based on their isoelectric point (pI). Novex™ pH 3-7 IEF Gels are used for pI determination and are excellent …

Instructions are provided below for electrophoresis of Novex® IEF Gels using the XCell SureLock ® Mini-Cell. For details, refer to the Novex ® Technical Guide available at

IEG 凝胶可用于快速检测蛋白质因脱氨基、磷酸化或糖基化而发生的微小变化，能够分离大小相似但无法在标准 SDS-PAGE 凝胶上分离的不同蛋白。 无需预聚焦。 总运行时间约为 2.5 小时 …

IEF can be performed using two techniques: immobilized pH gradients (IPG) with ampholytes covalently bound to a gel, or carrier ampholytes that migrate through a gel to generate the pH …