IEP is a reliable and accurate method for routine serum and urine protein evaluations, detecting both structural abnormalities and concentration changes. It has been widely used in identification of monoclonal gammopathies. Immunoelectrophoresis (IEP) combines two techniques, electrophoresis and immunodiffusion.

Bio-Rad offers a selection of agaroses suitable for IEP, IEF, and other specialty applications. Standard low, high, and zero -m r agarose are ideal for immuno-, counterimmunoelectrophoresis and IEF.

TITAN GEL Immunoelectrophoresis (IEP) is intended for semiquantita-tive protein determinations by immunoelectrophoresis. SUMMARY Immunoelectrophoresis (IEP) combines two techniques, electrophoresis and immunodiffusion. In this two-part procedure, proteins in a serum or urine sample are first separated according to charge by electrophoresis.

Immunoelectrophoresis is a general term describing many combinations of the principles of electrophoresis and reaction of antibodies, also known as immunodiffusion. [1] Agarose as 1% gel slabs of about 1 mm thickness buffered at high pH (around 8.6) is traditionally preferred for electrophoresis and the reaction with antibodies.

2018年11月14日 · Comparison of optimized IFE (TITAN Gel ImmunoFix, Helena) and IEP (TITAN Gel Immunoelectrophoresis, Helena) methods for immunotyping of a human serum paraprotein (IgG[κ]). Dots show sites of sample application; arrows indicate distortion (abnormal widening) of …

Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins based on their isoelectric point (pI). The pI is the pH at which a protein has no net charge and does not move in an electric field. Novex IEF Gels effectively create a …

IEP is a reliable and accurate method for routine serum and urine protein evaluations, detecting both structural abnormalities and concentration changes. It has been widely used in identification of monoclonal gammopathies. Helena offers both thick Titan IV agar and thin TITAN GEL IEP agarose for performing immunoelectrophoresis.

2015年12月7日 · IEP is a reliable and accurate method forroutine protein evaluations, detecting both structural abnormalities andconcentration changes. 1-6The most common application of IEP is the diagnosis of monoclonalgammopathies.

2024年4月3日 · Two-Dimensional Immunoelectrophoresis (2-D IEP), also known as crossed immunoelectrophoresis, is a powerful technique used for the quantitation and analysis of protein mixtures. This method involves two consecutive electrophoretic steps …

2022年7月5日 · When an electric current is applied to a slide layered with gel, the antigen mixture placed in wells is separated into individual antigen components according to their charge and size. Following electrophoresis, the separated antigens are reacted with specific antisera placed in troughs parallel to the electrophoretic migration and diffusion is ...