2022年6月22日 · We developed an automated two-color single-molecule tracking approach to dissect the oligomerization of tagged endogenous human IRE1 in live cells. In contrast to previous models, our data indicate that IRE1 exists as a constitutive homodimer at baseline and assembles into small oligomers upon ER stress.

2022年6月22日 · In this study, Belyy et al., have developed a powerful new imaging system--one that will benefit others in the cell biology community--to measure how a key transducer of the unfolded protein response , Ire1, responds to endoplasmic reticulum (ER) stress.

2020年2月4日 · In PNAS, Belyy et al. employ advanced quantitative imaging strategies to reveal the intricacies of the regulation of IRE1 during ER stress in the cellular context. The most conserved of the three UPR sensors is IRE1, which is present in …

Protein folding homeostasis in the endoplasmic reticulum (ER) is regulated by a signaling network, termed the unfolded protein response (UPR). Inositol-requiring enzyme 1 (IRE1) is an ER...

2024年2月8日 · Inositol-requiring enzyme 1 (IRE1) is a bifunctional kinase/RNase that is regulated by oligomeric transitions. When activated, mammalian IRE1 splices a specific mRNA encoding the transcription factor X-box binding protein (XBP1), which in turn helps reduce the load of misfolded proteins in the endoplasmic reticulum.

2021年9月29日 · We developed an automated two-color single-molecule tracking approach to dissect the oligomerization of tagged endogenous human IRE1 in live cells. In contrast to previous models, our data indicate that IRE1 exists as a constitutive homodimer at baseline and assembles into small oligomers upon ER stress.

2021年9月30日 · We developed an automated two-color single-molecule tracking approach to dissect the oligomerization of tagged endogenous human IRE1 in live cells. In contrast to previous models, our data indicate that IRE1 exists as a constitutive homodimer at baseline and assembles into small oligomers upon ER stress.

2024年2月8日 · One of the most conserved ER regulators is inositol-requiring enzyme 1 (IRE1), an ancient ER membrane-resident stress sensor that initiates a potent transcriptional response via its kinase/RNase domain when it detects a buildup of unfolded proteins in the ER lumen.

2021年12月15日 · Inositol requiring enzyme 1 (IRE1) mitigates endoplasmic-reticulum (ER) stress by orchestrating the unfolded-protein response (UPR). IRE1 spans the ER membrane, and signals through a cytosolic...

2020年1月21日 · The endoplasmic reticulum (ER) membrane-resident stress sensor inositol-requiring enzyme 1 (IRE1) governs the most evolutionarily conserved branch of the unfolded protein response. Upon sensing an accumulation of unfolded proteins in the ER lumen, IRE1 activates its cytoplasmic kinase and ribonuclea …