New insights on human IRE1 tetramer structures based on …
2020年10月15日 · Experimental evidence suggests that IRE1 forms several three dimensional (3D) structural variants: dimers, tetramers and higher order oligomers, where each structural …
Endoplasmic reticulum stress activates human IRE1α through
2022年6月22日 · Applying this approach to a key regulator of cellular proteostasis, IRE1, we demonstrated that the dimer-to-oligomer transition serves as the primary regulatory step in …
Activation of the IRE1 RNase through remodeling of the kinase …
2020年12月14日 · Excess unfolded proteins in the ER lumen induce dimerization and oligomerization of IRE1, triggering kinase trans-autophosphorylation and RNase activation. …
Sensor dimer disruption as a new mode of action to block the IRE1 ...
2022年1月1日 · The findings herein, showing the existence and activity of IRE1 dimer disruptors, opens for an entirely new mode of action to block the UPR, and can serve as an additional and …
How IRE1 Reacts to ER Stress - Cell Press
2008年1月11日 · IRE1-mediated cleavage of HAC1 mRNA depends on its kinase activity—only wild-type kinase-active IRE1 is able to cleave its mRNA substrate in vitro. ER stress promotes …
Structure and Intermolecular Interactions of the Luminal …
2003年5月16日 · IRE1α is a type-I transmembrane glycoprotein for which the N-terminal luminal domain (NLD) senses the accumulation of unfolded proteins. Previously we demonstrated that …
Molecular mechanisms of human IRE1 activation through …
IRE1 transduces the unfolded protein response by splicing XBP1 through its C-terminal cytoplasmic kinase-RNase region. IRE1 autophosphorylation is coupled to RNase activity …
The crystal structure of human IRE1 luminal domain reveals a ... - PNAS
2006年9月26日 · Structure of the IRE1 NLD Dimer. The NLD forms stable dimers in vitro with an apparent molecular mass of 96 kDa ( 24 ). Because the asymmetric unit of the crystal contains …
Structure of the Ire1 autophosphorylation complex and …
We have now determined the crystal structure of the cytoplasmic kinase-RNase region of human Ire1α at 2.7 Å resolution, in a dephosphorylated state, and in a complex with Mg 2+-ADP, …
The unfolded protein response signals through high-order assembly of Ire1
2008年12月14日 · A recent crystal structure of the kinase/RNase domain of Ire1 reveals a two-fold symmetric dimer with a back-to-back arrangement of the kinase domains, compactly …
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