Longa method, cerebral infarct volume, and HE staining were used to evaluate the efficacy of GRb1 in mice with a mouse model of middle cerebral artery occlusion reperfusion (MCAO/R). We also investigated the effect and mechanism of GRb1 against ischemic stroke using in vitro oxygen-glucose deprivation reperfusion (OGD/R) model.

2023年3月1日 · In our study, the high expression levels of GRP78, p-PERK, p-IRE1, and ATF6 were observed in cortical tissue of rats in MCAO group, indicating the activation of ER stress induced by I/R injury. However, pre-ischemic EE successfully restrained the growing trend of these hallmark proteins of ER stress.

2024年3月1日 · ERS overactivation activates both autophagy and apoptosis, the two pathways of cell death, and triggers cell death. Among them, IRE1 mediates the phosphorylation of the anti-apoptotic B-cell lymphoma-2 (Bcl-2) by initiating one of the upstream regulatory components of autophagy, the JNK signaling pathway .

Endoplasmic reticulum (ER) is the main organelle for protein synthesis, trafficking and maintaining intracellular Ca 2+ homeostasis. The stress response of ER results from the disruption of ER homeostasis in neurological disorders. Among these disorders, cerebral ischemia is a prevalent reason of death and disability in the world.

2015年8月6日 · To determine whether IRE1 pathway can be affected, we tested the expression of p-IRE1 (activated form of IRE-1) in the penumbra of the infarct of MCAO rats with and without treatment. The results showed that phosphorylated IRE1 is highly expressed in the penumbra of the infarct in the MCAO rat brain in comparison to the sham-operated group.

2023年6月21日 · IRE1 evokes neuronal apoptosis by activating c-Jun NH2-terminal kinase (JNK), while PERK and ATF6 may also trigger cell apoptosis via the C/EBP homologous protein (CHOP) pathway 9,10.

2024年3月4日 · Protein expression of IRE1, PERK, ATF6, and GRP78 was detected after MCAO/R as well as iTBS intervention. The results showed that MCAO/R rats had higher levels of IRE1, PERK, ATF6, and GRP78 compared to the sham-operated group, and the levels of IRE1, PERK, ATF6, and GRP78 were reduced in MCAO/R rats treated by iTBS (Fig. 1 A-H).

2020年11月10日 · Acupuncture treatment decreased infarct volume, neurological score and suppressed ER stress via inactivation of ATF-6, PERK, and IRE1 pathways in MCAO rats. Attributing to ER stress suppression, 4-PBA (ER stress inhibitor) promoted the beneficial effect of acupuncture against cerebral I/R injury.

Here, we report results from an experimental study designed to evaluate the potential of the IRE1/XBP1/HBP/O-GlcNAc axis as a target for neuroprotection in ischemic stroke. Using genetic and pharmacologic approaches, we provide evidence that the IRE1/XBP1 UPR pathway critically defines ischemic stroke outcome.

2021年2月9日 · LTC treatment alleviated OGD/R induced ER stress by inhibiting the activation of protein kinase RNA (PKR)-like ER kinase (PERK)/eukaryotic translation initiation factor 2 (eIF2α) and inositol requiring enzyme 1 (IRE1)/tumor necrosis factor receptor-associated factor 2 …