2021年6月3日 · Three ER-resident transmembrane proteins orchestrate the UPR: inositol-requiring enzyme 1 (IRE1), activating transcription factor 6 (ATF6), and protein kinase RNA (PKR)-like ER kinase (PERK)....

2022年5月6日 · Activated IRE1α cleaves XBP1 mRNA, a key step in production of the transcription factor XBP1s. In addition, IRE1α cleaves select mRNAs through regulated IRE1α-dependent decay (RIDD). Accumulating...

For instance, RIDD cleaves RNA at an XBP1-like consensus site but with an activity divergent from XBP1 mRNA splicing and can either preserve ER homeostasis or induce cell death. Here we review recent findings on RIDD and propose a model of how IRE1 RNase activity might control cell fate decisions.

2022年10月4日 · PIDDosome ablation or IRE1 inhibition blunt steatohepatitis and diminish INSIG2 expression. Conversely, while inhibiting simple steatosis, SCAP ablation amplifies IRE1 and PIDDosome activation and liver damage in NASH-diet-fed animals, effects linked to ER disruption and preventable by IRE1 inhibition.

在TNFR-1介导的凋亡通路中，三聚化的TNFR-1募集接头蛋白TRADD，TRADD通过自身的DD可汇聚FADD、RIP和TRAF-2。 这里分2条通路：（1）当结合的是FADD，便导致Caspase-8前体寡聚化，引发Caspase级联反应和凋亡；（2）当结合的是RIP，便激活TRAF-2，形成TRADD-RIP-TRAF-2复合物，通过磷酸化进一步激活NF-κB诱导激酶（NIK），NIK活化IκB激酶（IKK），IKK磷酸化IκB，导致NF-κB的降解和释放，NF-κB转位至细胞核发挥激活转录的作用，主要激活一些 …

2014年11月6日 · IRE1, an endoplasmic reticulum (ER) kinase/RNase, splices XBP1/HAC1 to produce an active transcription factor but also cleaves ER-associated mRNA through regulated IRE1-dependent decay (RIDD). Using biochemical studies, Tam et al. demonstrate that cleavage of XBP1 and HAC1 and RIDD are distinct and separable activities.

内质网是细胞内能够精细调控细胞生长、存活及功能的膜系统,是促进细胞内环境稳定的重要细胞器.内质网应激(ERS)中的感应器IRE1具有促细胞存活及促凋亡活性,这主要归因于XBP1 mRNA剪切活性及RIDD (regulated IRE l-dependent decay)活性.RIDD能特异性识别“XBP1样位点”,识别 ...

2014年5月1日 · Inositol-requiring enzyme 1 (IRE1) is the most conserved transducer of the unfolded protein response (UPR), a homeostatic response that preserves proteostasis. Intriguingly, via its endoribonuclease activity, IRE1 produces either adaptive or death signals.

2022年5月6日 · Activated IRE1α cleaves XBP1 mRNA, a key step in production of the transcription factor XBP1s. In addition, IRE1α cleaves select mRNAs through regulated IRE1α-dependent decay (RIDD). Accumulating evidence implicates IRE1α in the regulation of lipid metabolism. However, the roles of XBP1s and RIDD in this process remain ill-defined.

In this study, we employed unbiased lipidomic and tran-scriptomic approaches to investigate the contribution of IRE1 α signaling to lipid metabolism in TNBC. The integration of these data enabled...