
eIF2α-CHOP-BCl-2/JNK and IRE1α-XBP1/JNK signaling promote …
2019年11月26日 · Our study demonstrates that the induction of eIF2α-CHOP-BCL-2/JNK and IRE1α-XBP1/JNK signaling cascades promote apoptosis and cytokines secretion, and these signaling cascades support NDV...
详解内质网应激/未折叠蛋白反应三条信号通路 - 简书
2020年8月8日 · IRE1α首先发生磷酸化,并形成二聚体。 p-IRE1α接下来会催化一个重要的过程:使Xbp1 mRNA发生剪切,形成一个功能活跃的转录因子Xbp1s。 Xbp1s进入细胞核后会启动一系列基因转录 (如脂质合成、ERAD等),从多个层次缓解内质网应激。 除此之外p-IRE1α可以通过RIDD过程促进mRNA的降解,减缓新生多肽链合成,降低内质网压力。 (这个过程是不是很像学校(内质网)丧心病狂的把幼儿园的小孩(mRNA)全灭了,来年肯定没有新生(多肽链)入 …
Science | 发现全新的决定内质网应激下细胞命运的调控因子 - 知乎
后续的研究证明,QRICH1是属于UPR PERK-eIF2a通路的关键效应因子,其翻译受到QRICH1 mRNA上游开放阅读框的调控。 通过结合RNA-seq和染色质免疫沉淀测序 (ChIP-seq)的结果,研究人员发现 QRICH1作为转录调节因子,结合启动子区域控制转录模块,调控蛋白质翻译和分泌网络。 QRICH1介导的翻译激活增加了进入内质网的蛋白通量和蛋白毒性,而QRICH1敲除可保护肠上皮细胞免受蛋白毒性。 最后,为了评估QRICH1在人类疾病中的作用,研究人员分析了 溃疡 …
eIF2α phosphorylation is pathognomonic for immunogenic cell …
2018年1月22日 · Active IRE1 possesses in its cytoplasmic domain an endoribonuclease function allowing it to excise an intron of 26 nucleotides from the mRNA of the transcription factor X box-binding protein 1...
The eIF2 kinase PERK and the integrated stress response ... - PubMed
Three ER stress sensors (PERK, ATF6, and IRE1) implement the UPR. PERK phosphorylation of the α subunit of eIF2 during ER stress represses protein synthesis, which prevents further influx of ER client proteins.
Novel mechanism of enhancing IRE1α-XBP1 signalling via the …
2016年4月7日 · In this study, we found that IRE1α was induced by ER stress both in vivo and in cultured cells. Using various knockout cells, we revealed that the increase in IRE1α expression depended on PERK-ATF4...
To prevent a further increase in protein-folding demand in the ER, PERK tran-siently inhibits general protein translation through phos-phorylation of eukaryotic initiation factor 2 alpha (eIF2a). Phosphorylated elF2a can also selectively activate trans-lation of mRNAs including the ATF4 transcription factor to regulate UPR target genes [27].
Role of eIF2α Kinases in Translational Control and Adaptation to ...
Key themes in the review will be the mechanisms regulating eIF2α kinases, the role that upstream open reading frames (uORFs) play in translational control, and the impact that altered P-eIF2α levels by gene mutations or small molecule inhibitors can have on health and disease.
内质网应激相关激酶PERK有什么重要作用?(Cancer Cell)
2022年11月24日 · 蛋白激酶R(PKR)样内质网激酶(PERK)是四种已知的激酶之一,通过激活真核细胞起始因子2α(eIF2α)或其他信号转导级联来响应细胞应激。 PERK在胰腺β细胞中高度表达,对β细胞的发育、分化和功能至关重要。 PERK是具有丝氨酸/苏氨酸细胞质结构域的I型ER跨膜受体。 分子伴侣(如BiP/GRP78)在维持PERK管腔结构域的激酶活性处于不活跃状态中起着至关重要的作用。 内质网应激条件下,BiP/GRP78蛋白与PERK分离,导致应激敏感PERK结构 …
The eIF2 kinase PERK and the integrated stress response facilitate ...
2011年9月14日 · ER stress activates three transmembrane proteins—ATF6, IRE1 (ERN1), and PERK (PEK/EIF2AK3)—which together induce the unfolded protein response (UPR), involving both transcriptional and translational regulation of genes that serve to expand the processing capacity of the ER and return this organelle to homeostasis (Ron and Walter, 2007; Wek ...