2013年9月17日 · We found in macrophages that IRF3, ERK MAP-kinases, and PKR are essential to NO production in response to RNA-virus mimic, poly I:C, a TLR3 agonist. ERK’s role in NO induction may be through phosphorylation of serine-171 of IRF3 and expression of NO-inducing cytokines, IL-6 and IFN-β.

2018年7月1日 · We examined whether inhibition of ERK activity blocked IRF3-mediated immune responses. ERK1 and ERK2 isoforms can have different cellular functions; thus, to determine which ERK isoform is involved in the regulation of IRF3 activity, we performed siRNA to knockdown either ERK1, ERK2, or both in RAW 264.7 macrophages and saw 70% knockdown of ...

2024年2月21日 · We found that DUSP4 regulates the activation of TBK1 and ERK1/2 in a signalling complex containing DUSP4, TBK1, ERK1/2 and IRF3 to regulate the production of type I IFNs. Mice deficient in DUSP4...

MAPK通路有4种主要的分支路线： ERK 、 JNK 、 p38 /MAPK和ERK5。其中，JNK和p38功能相似，跟炎症、凋亡、生长都有关；ERK主要管细胞生长、分化，其上游信号是著名的 Ras/Raf 蛋白。且分支路线所使用3种激酶都是不同的，可作为通路中的Biomarker。

TANK 可将上游 RLR 信号转导连接至 TANK 结合激酶 1 (TBK1)，TBK1 会诱导干扰素调节因子 3 (IRF-3) 的磷酸化。 IRF-3 磷酸化和随后的二聚化会诱导 IRF-3 核转位，IRF-3 随后结合干扰素刺激应答元件 (ISRE)，从而导致 I 型干扰素基因表达。 胞质双链 DNA 检测也通过多个入口流入 TBK1-IRF-3 信号转导轴。 主要的胞质 DNA 结合感应蛋白是环 GMP-AMP 合成酶 (cGAS)，cGAS 通过产生环二核苷酸 c-GMP-AMP (cGAMP) 对 DNA 结合产生反应。 cGAMP 结合 STING 并激活 …

2016年12月14日 · A mechanistic study showed that scoparone specifically inhibited the LPS-stimulated activation via a major regulation of IRF-3 and a regulation of ERK, whereby the phosphorylation in the BV-2 microglial cells is blocked.

2009年1月20日 · Interferon regulatory factor (IRF)3 is critical for the transcriptional induction of chemokines and cytokines during viral or bacterial invasion. The kinases...

2020年12月15日 · This study investigated responses to Toll-like receptor 2 (TLR2)-driven extracellular signal-related kinase (ERK) signaling in dendritic cells (DCs) versus macrophages. TLR2 signaling was induced with Pam₃Cys-Ser-Lys₄, and the role of ERK signaling was interrogated pharmacologi …

Calcium influx suppresses interferon regulatory factor-1 (IRF-1) induction via an Erk mitogen-activated protein kinase (MAPkinase)-dependent mechanism. Erk-independent negative regulation of p35 may occur via suppression of the nuclear factor (NF)-κB pathway.

ERK expression is critical for development and their hyperactivation plays a major role in cancer development and progression. The Ras/Raf/MAPK (MEK)/ERK pathway is the most important signalling cascade among all MAPK signal transduction pathways, and plays a crucial role in the survival and development of tumour cells.