Interferon-stimulated gene (ISG) products take on a number of diverse roles. Collectively, they are highly effective at resisting and controlling pathogens. In this review, we begin by introducing interferon (IFN) and the JAK-STAT signaling pathway to …

ISG15, the Mx proteins, the 2′,5′-oligoadenylate synthetase (OAS)-directed RNaseL pathway and PKR have varying responsiveness to type I IFNs: ISG15 is one of the most highly induced ISGs and ...

2022年4月14日 · OAS1, a top-ranked hit across multiple screens, exhibited strong antiviral effects against SARS-CoV-2, which required OAS1 catalytic activity. These studies demonstrate a high-throughput approach to assess antiviral functions within the ISG repertoire, exemplified by identification of multiple SARS-CoV-2 restriction factors.

The OAS/RNase L pathway was one of the first ISG antiviral mechanisms to be identified and elucidated in the 1970s [reviewed in (61–64)]. Binding to dsRNA activates OAS, which then synthesizes 2’-5’ oligoadenylates (commonly abbreviated as 2-5A) that in turn activate RNase L to cleave cytoplasmic RNA, thereby inhibiting viral replication ...

Many viruses trigger the type I interferon (IFN) system, leading to the transcription of hundreds of interferon-stimulated genes (ISGs). The products of these ISGs exert numerous antiviral effector functions, many of which are still not fully described.

2018年3月1日 · 摘要：干扰素刺激基因（Interferon-stimulated genes，ISGs）作为由干扰素（Interferons，IFNs）诱导表达的基因，在宿主抵抗病毒感染的过程中发挥着至关重要的作用。 越来越多的研究表明，ISGs能够靶向病毒复制的不同阶段进而抵抗病毒感染。 由于ISGs成员众多，且各自的结构及其在细胞中的定位也各不相同，这决定了ISGs在宿主体内以不同机制来发挥抗病毒作用。 本文将简要介绍IFNs如何通过JAK-STAT通路调控ISGs的表达，并归纳和讨论不 …

ISGs包括PKR；2’-5'-OAS 和RNAse L。PKR识别ds RNA，然后关闭蛋白表达（PKR识别dsRNA被激活，使eIF2磷酸化，然后其不能发挥翻译起始作用）。OAS作用（OAS被dsRNA激活，然后激活RNAse L, 可以降解宿主和 病毒RNA ，终止蛋白翻译）。

2015年6月1日 · Oligoadenylate synthetases (OAS) belong to a family of ISGs characterized by their ability to synthesize 2′–5′ oligoadenylates, which induce RNA degradation by activating a latent RNase, RNase L [17].

2020年4月24日 · We find that IFN and ISGs induced by transfected DNA through the cGAS pathway severely suppress expression levels of transfected genes. Particularly, mobilization of the OAS-RNaseL system is a key factor in restraining transgene expression.

2024年3月10日 · isg抗病毒功能的一个共同机制是通过双链rna激活的酶 （如pkr、oas、adar等） 抑制蛋白翻译，ifn诱导的 寡腺苷酸合成酶1 （ oas1 ）可以结合病毒双链rna，并被激活，利用atp合成短的2’-5’寡腺苷酸 （2-5a） ，这些分子可以结合并激活rnase l，从而降解病毒rna和细胞 ...