The present protocol outlines the generation of HFD-fed mice as well as two frequently used methods, the oral glucose tolerance test (OGTT) and the insulin resistance test (ITT), which …

2020年11月8日 · 相反地，如果外源给予胰岛素，检测特定时间段内的血糖水平 ，就是胰岛素耐量实验（「insulin tolerance test, insulin-induced hypoglycemia test」）简称为 「ITT」, 而外源 …

2020年8月20日 · Together, these data demonstrate significant differences in HFD- and WD-induced adiposity in C57BL/6J-related substrains, which should be considered in the design of …

而24周高脂饮食（HFD）实验中，敲基因小鼠体重和体脂都低于WT组（图1a-c）。 敲基因小鼠，BAT、iWAT和eWAT和肝脏在内的主要脂肪存贮组织的重量抑制下降（图1d、e）。 组织学 …

2020年11月8日 · 相反地，如果外源给予胰岛素，检测特定时间段内的血糖水平，就是胰岛素耐量实验（「insulin tolerance test, insulin-induced hypoglycemia test」） 简称为 「ITT」, 而外 …

2023年12月21日 · Hepatocyte-specific Bach1 deletion in male mice on a high-fat diet (HFD) ameliorates hyperglycemia and insulin resistance, improves glucose homeostasis, and …

At 24 wk of age, i.e., after 19 wk of diet, the HFD male ITT responses continued to indicate a greater loss of insulin sensitivity compared to other groups; at this time, HFD females remained...

2023年3月17日 · To induce insulin resistance, 6-week-old male mice were fed on a 60% high fat diet (HFD) for 10 weeks to develop insulin resistance. Total body weight was monitored …

We illustrate in detail the oral glucose tolerance test (OGTT), which monitors the peripheral disposal of an orally administered glucose load and insulin secretion over time. Additionally, …

2018年1月7日 · The presented protocol describes well-established methods for the generation of HFD mice, a robust animal model used for metabolic research, as well as the conduction of …