2020年11月8日 · 相反地，如果外源给予胰岛素，检测特定时间段内的血糖水平 ，就是胰岛素耐量实验（「insulin tolerance test, insulin-induced hypoglycemia test」）简称为 「ITT」, 而外源性胰岛素的给予方式，是通过腹腔注射来实现的；如果出现了胰岛素不敏感，在注射胰岛素之后，血糖 ...

2021年6月11日 · Nature Metabolism - The two most common tests for determining metabolic health in mice are the glucose tolerance test (GTT) and insulin tolerance test (ITT). GTTs and ITTs are inexpensive and...

Here, we provided an easy to follow very detail protocol that allows the performance of GTT and ITT in mice. It is simple to implement and can be quickly used by skilled scientists. Furthermore, we specify some important factors that should be taken into account, including sex-, age-, and strain-related differences and include some practical ...

GTTs and ITTs in mice: simple tests, complex answers Nat Metab. 2021 Jul;3(7):883-886. doi: 10.1038/s42255-021-00414-7. Authors Sam Virtue 1 , Antonio Vidal-Puig 2 3 Affiliations 1 University of Cambridge Metabolic Research Laboratories, Institute ...

Insulin Tolerance Test (ITT) – A test performed to evaluate insulin sensitivity by monitoring glucose clearance from the blood over time following administration of an injection of insulin. Poor sensitivity to insulin results in a reduced rate of glucose clearance.

2024年11月25日 · Glucose tolerance tests (GTT) and insulin tolerance tests (ITT) are by far the most common and simple metabolic tests used by researchers to assess glucose homeostasis and...

1. The evening before the ipITT test, weight all mice and fast overnight (16-18h) by transferring mice to clean cages with no food. NOTE: Ensure access to drinking water. 2. On the morning of the ipITT, lace the mouse in a duplex cage with mouse of same sex. NOTE: no food in cage but access to water is critical. Order cages in the order of ...

The Insulin Tolerance Test (ITT) is designed to determine the whole body sensitivity of insulin receptors by measuring blood glucose levels changes before and after insulin administration. This is a standard test to determine the insulin resistance status in humans and experimental animals.

2021年9月3日 · However, we recently reassessed the optimal fasting time needed before ITT during a fast in C57Bl6/J mice and demonstrated that a 2-hour fast during the photophase is sufficient to reduce blood...

In vivo metabolic tests are highly valuable to determine whether atherosclerosis progression in mouse models is accompanied by carbohydrate metabolism alterations such as glucose intolerance and insulin resistance. In this chapter, we describe protocols to perform in...