Assays for superoxide dismutase (SOD) and catalase (CAT) activities are widely employed to indicate antioxidant responses underlying the toxic effects of test chemicals. Yet, earlier studies mainly described the procedures as performed according to ...

2024年2月22日 · 超氧化物歧化酶（sod）是生物体内的重要抗氧化酶，其主要功能是催化超氧阴离子（o2-）转化为过氧化氢（h2o2）和氧气（o2）。 在细胞内，超氧阴离子是一种具有高度活性的活性氧，可以攻击细胞膜和线粒体等细胞结构，导致细胞损伤。

There are three major types of primary intracellular antioxidant enzymes in mammalian cells - SOD, catalase, and peroxidase, of which glutathione peroxidase (GPx) is the most prominent. The SODs convert O 2•- into H 2 O 2, while the catalases and peroxidases convert H 2 O 2 into water.

2023年1月1日 · Intracellular SOD/CAT activities were regulated by TCC-induced oxidative stress. In vitro SOD/CAT activities were interfered through the binding of TCC. TCC affected the structure, aggregation behavior and active center in SOD/CAT. TCC binding to SOD/CAT was primarily mediated via multiple interactions.

2022年4月1日 · Herein, we designed a pluronic-based nanocarrier loaded with dual antioxidant enzymes present in vivo (superoxide dismutase (SOD) and catalase (CAT)) as a nanoreactor system for the regeneration of inflammatory tissue. The catalytic activity of each enzyme was enhanced by loading it into the nanocarrier.

2023年12月1日 · This paper describes a simple and cost-effective method to determine SOD content and CAT activity in mammalian cell cultures following exposure to environmental chemical mixtures by measuring absorbance in 96-well microplates.

SOD能催化超氧阴离子转化为H2O2和O2的反应,是生物体内清除自由基的首要物质,CAT和POD是清除H2O2的酶,三者通过协同作用维持植物体内的自由基含量保持稳态水平,防止由于自由基引起的植物生理生化上的改变. 超氧化物歧化酶,是生物体内重要的抗氧化酶,广泛分布于各种生物体内,如动物,植物,微生物等.SOD具有特殊的生理活性,是生物体内清除自由基的首要物质.SOD在生物体内的水平高低意味着衰老与死亡的直观指标；现已证实,由氧自由基引发的疾病多达60多种.它可对 …

2018年11月7日 · This genotype also had higher catalase (CAT), superoxide dismutase (SOD), remarkable and dramatic increment in ascorbate-glutathione content, ascorbate-glutathione redox and...

2023年8月22日 · cat类活动 通过溶解氧计测量H2O2的产氧量来评估CAT样活性。 简单地说，将终浓度为材料(100µg mL−1)的材料与终浓度为5mM的H2O2在5ml水中混合。室温下反应30min后，将反应液离心除去物料，用溶解氧计(JPB-607A, INESA科学仪器有限公司，中国)检测 上清液 中的溶 …

通过深入的数据分析和合理的结果解释，可以更好地理解抗氧化酶（sod、pod、cat）活性测定的意义和价值。 同时，为后续的研究提供科学依据和指导方向。