2011年11月1日 · iCIEF is a more suitable technique to assess charge heterogeneities of ADCs. The separation mechanism of this electrophoretic technique is based primarily on pI differences of protein charge variants.

Having been amply described already in 1960-1961, how comes that isoelectric focusing (IEF) went largely unnoticed for just about a decade? Well Harry was a free-lance scientist in a …

Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different charged molecules by differences in their isoelectric point (pI). [1][2] It is a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest is a function of t...

Experience icIEF separation and UV detection coupled with mass spectrometry identification on the ZenoTOF 7600 system. One workflow, on a single platform, enables rapid monitoring of intact biotherapeutics and identification of charge variants throughout the drug development pipeline.

icIEF is a technique to separate proteins based on their isoelectric point. See how Maurice an automated icIEF instrument can simplify protein charge analysis.

To support analysis of the charge heterogeneity of USP mAbs, we developed methods for cIEF using the SCIEX platform (PA 800 Plus) and icIEF using Bio-Techne’s Protein Simple Maurice and iCE3 platforms (Table 3).

imaging capillary isoelectric focusing (icIEF) for charge heterogeneity analysis of an Antibody Drug Conjugate (ADC) and its corresponding antibody intermediate.

Why is Peak Identification Needed for icIEF? Pfizer has a portfolio of divergent and complex modalities For AAV, capsid deamidation is a CQA. An icIEF method was able to be developed; however, it is unclear which peaks represent which capsid proteins and/or acidic species thereof Assigning icIEF peaks was a complex, multiyear process and

BioPharmaSpec offers Imaging Capillary Isoelectric Focusing (icIEF) and Ion Exchange Chromatography as methods for assessing charge isoform pattern.

Iso-electric focusing (IEF) has proven to be a very versatile separation method for the characterisation, identification, purity determination, and quantitation of amphoteric substances such as peptides and proteins.