icief（毛细管等电聚焦电泳）是一种用于分析蛋白质电荷异构体的高效技术。通过icief，研究者可以精确地分离和鉴定具有不同等电点（pi）的蛋白质或肽段。本文将介绍icief的检测原理、与iec（离子交换色谱）的区别、…

2025年3月1日 · We present an integrated protocol for studying the charge heterogeneity of therapeutic proteins combining two workflows: imaged capillary isoelectric focusing (icIEF) with fractionation and online mass spectrometry (MS) detection on a single platform.

This review discusses recent developments in icIEF technology, including: (i) development of high-resolution carrier ampholytes, pI markers, and capillary coatings to optimize separation resolution; (ii) the development of icIEF fractionation, enabling icIEF peak collection in quantities suitable for downstream analyses such as peptide mapping ...

2011年11月1日 · iCIEF separation is conducted by focusing isoforms in an ampholytic pH gradient through an applied electric field. Following the focusing step is a detection step, which in conventional CIEF would entail a chemical or pressure mobilization across a fixed UV detector 4,.

2022年5月1日 · Capillary isoelectric focusing with whole-column imaging detection (icIEF) technology is now the mainstream technology to perform analytical isoelectric focusing analysis in pharmaceutical industry.

The Maurice instrument accelerates protein charge analysis by automating several steps, allowing icIEF method development in a day while providing high-quality data.

2018年3月25日 · 结论： iCIEF作为一种新的技术手段，用于重组蛋白类药物电荷异质性及等电点分析，方法快速、准确，重复性好，能够较好地检测高度糖基化的重组VEGFR：Fc-融合蛋白产品的电荷异质性，为保障Fc-融合蛋白类产品生产工艺的稳定性及质量控制提供了一种快速、可靠的分析方法。 Abstract: Objective: To develop an imaged capillary isoelectric focusing electrophoresis method for charge heterogeneity analysis of recombinant human IgG VEGFR:Fc-fusion protein.

icIEF具有方法开发简单、通量高、等电点(pI)测定和定量重现性好、易于开发平台方法等优点。 去除了传统clEF迁移步骤，将分析时间从40到60分钟减少到每个样品5到15分钟。

2025年2月19日 · Recognized as a gold standard by the industry, icIEF leverages a pH gradient to provide high-resolution profiling of charge variants in biotherapeutics. In this review, critical experimental parameters for icIEF method development in the context of biotherapeutic drug development and QC will be discussed.

2023年2月5日 · We demonstrate how this methodology proves highly-sensitive and highly reliable for the characterization of commercial monoclonal antibodies (mAbs) and antibody-drug-conjugates (ADCs). The whole workflow of icIEF-MS for protein heterogeneity is straight forward and accurate and can be performed within 45 min.