Identification of bacteria using mass spectrometry techniques
2013年11月1日 · In this review, we summarize both common and rare biomarkers used in bacteria identification. We summarize developments and innovations in mass spectrometry methods applied to bacteria identification over the last decade, and compare current commercial systems designed for clinical use.
Identification of Microorganisms by Liquid Chromatography-Mass ...
2020年12月1日 · A LC–MS compatible sample preparation method was developed that enabled accurate identification of bacteria grown in blood culture flasks to species level based on LC-MS 2 bottom-up proteomics, database searches and matching …
Identification of Pathogens by Mass Spectrometry - PMC
Direct analysis of whole pathogenic microbial cells using MS without sample fractionation reveals specific biomarkers for taxonomy and provides rapid and high-throughput capabilities.
BacteriaMS - Analysis of bacteria by MALDI-TOF mass spectrometry
Identification of bacteria in a MALDI-TOF mass spectrum. Baseline correction, intensity normalization and peak extraction. Compare two peak lists and calculate the similarity between them. Species identification by spectral pattern matching with statistical assessment. Cluster analysis of different bacterial spectra based on similarity scores.
MALDI-TOF Mass Spectrometry for Microorganism Identification
2015年1月1日 · Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid, accurate and high-throughput method for microorganism identification. MALDI-TOF MS identification of microorganisms has revolutionised the clinical microbiology laboratory offering species-level identifications in minutes with accuracy that ...
Mass spectrometry-based Methods for the Identification of Bacteria…
We performed an extensive search using electronic databases, including PubMed, as well as additional sources relevant to mass spectrometry (MS) and bacterial identification. Keywords used in the search included mass spectrometer, microbiology, bacteria, identification, and detection.
MALDI-TOF MS for Bacterial Identification | SpringerLink
2022年4月15日 · MALDI-TOF MS has proved its effectiveness to show the more accurate nutritionally variant streptococci (NVS), including Granulicatella and Abiotrophia species. The speedy process with MALDI-TOF MS-based identification and antibiotic susceptibility result quickly guides the further treatment.
Application of MALDI-TOF MS to rapid identification of anaerobic bacteria
2019年11月7日 · Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification. To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes.
Identification of Microorganisms by Liquid Chromatography …
In this proof of concept study, we present an alternative bottom-up proteomics method for microbial identification. The proposed approach involves efficient extraction of proteins from cultivated microbial cells, digestion by trypsin and LC-MS measurements.
Sample preparation and culture condition effects on MALDI‐TOF MS …
2021年10月13日 · Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is an excellent tool for bacterial identification. It allows high throughput, sensitive and specific applications in clinical diagnostics and environmental research.