2024年9月13日 · Two-dimensional [1 H, 15 N]-HSQC spectrum of uniformly 15 N/ 13 C-labelled sdAb-mrh-IgG at 25 o C in 20 mM sodium phosphate pH 6.5, 150 mM NaCl, 3 mM NaN 3 and 10% (v/v) D 2 O. The labels show the assigned HN from the backbone and side-chain of the amino acid residues. Stars identify residues with two backbone amide HN peaks

2009年6月12日 · In order to characterize the folding pathway of the intrinsically disordered C H 1 domain, 15 N-HSQC spectra were recorded at 12.5°C every 14 min immediately after adding unlabeled C L to 15 N-labeled C H 1 by using selective …

The results show that 2D 1 H- 13 C HSQC NMR is incredibly sensitive to primary, secondary, tertiary, and quaternary structures, and provides unique fingerprints for both the IgG1 and IgG2 subclasses used.

2024年1月1日 · In the present study, we exploit the advantages of the 1 H– 13 C HSQC experiment to determine tetrad-G’s glycosidic conformation and thus folding topology of G-quadruplexes. We use several examples to demonstrate the clear and straightforward determination of the guanine glycosidic conformations and G-quadruplex folding structures.

2024年8月29日 · The single-domain antibody examined in this study, sdAb-mrh-IgG, was engineered to recognize IgG from rats, mice, but it also weakly recognizes IgG from humans (Pleiner et al. 2018). A search of the Protein Data Bank revealed only one NMR structure of a single-domain antibody, which is unrelated to sdAb-mrh-IgG.

2021年5月5日 · The results show that 2D 1 H- 13 C HSQC NMR is incredibly sensitive to primary, secondary, tertiary, and quaternary structures, and provides unique fingerprints for both the IgG1 and IgG2 subclasses used.

The 2D 1 H- 13 C HSQC spectral comparison of insulin glargine DPs provided similarity metrics for chemical shift difference (Δδ) and methyl peak profile, i.e., 4 ppb for 1 H, 15 ppb for 13 C and 98% peaks with equivalent peak height. Finally, 2D 1 H- 15 N sofast HMQC was demonstrated as a sensitive method for comparison of small protein HOS.

In this chapter, the main purpose is to show and explain the different HSQC-based strategies by designing pulse sequences for turning standard 2D 1H-13C HSQC into an “intrinsically” quantitative version.

多糖的结构解析中，HSQC二维核磁是开展糖苷键连接确认的基础谱图。 从有机物的HSQC解谱而言，代表的相连接的C-H相关，比如乙醇CH3-CH2-OH，其HSQC谱图中，甲基的质子和甲基碳有相交叉的信号，而亚甲基质子和亚甲基…

2014年10月8日 · The Fc portion of immunoglobulin G (IgG) recruits complements and its cognate receptors, thereby promoting defensive mechanisms in the humoral immune system. These effector functions critically depend on N -glycosylation at the Fc region, which is therefore regarded as a crucial factor in the design and production of therapeutic antibodies.