The model that emerges to connect IL5RA transcription with eosinophilopoiesis involves complex and dynamic activity, including both P1 and P2 promoters, several interacting transcription factors, and both signaling and antagonistic protein products.

百奥动物建立了稳定的B-hIL5/hIL5RA小鼠模型，可用于哮喘等疾病的临床前研究和药理药效学评价。 蛋白表达分析. 通过ELISA分析纯合B-hIL5/hIL5RA小鼠中种属特异性IL5的表达。 收集野生型C57BL/6小鼠和纯合B-hIL5/hIL5RA小鼠，用抗CD3ε抗体和抗CD28抗体体外刺激，用种特异性IL5 ELISA试剂盒检测细胞培养上清液。 在野生型小鼠中可检测到小鼠IL5。 人IL5仅在纯合B-hIL5/hIL5RA小鼠中检测到，而在野生型小鼠中未检测到。 蛋白表达分析. 通过FACS分析纯 …

2021年9月23日 · The model that emerges to connect IL5RA transcription with eosinophilopoiesis involves complex and dynamic activity, including both P1 and P2 promoters, several interacting transcription factors, and both signaling and antagonistic protein products.

2024年12月25日 · IL5RA (Interleukin 5 Receptor Subunit Alpha) is a Protein Coding gene. Diseases associated with IL5RA include Pancreatic Vasoactive Intestinal Peptide Producing Tumor and T2-High Asthma. Among its related pathways are MAPK family signaling cascades and Interleukin-2 family signaling.

2024年7月9日 · We combined single-cell proteomics and transcriptomics and generated transgenic IL-5Rα reporter mice to revisit eosinophilopoiesis. We reconciled human and murine eosinophilopoiesis and provided extensive cell-surface immunophenotyping and transcriptomes at different stages along the continuum of eosinophil maturation.

2024年3月1日 · In present study, we employed single-cell analyses alongside molecular and cellular assays to unveil the expression of IL5RA in lung epithelial cells. Moreover, using both in vitro and in vivo models, we demonstrated a notable upregulation of epithelial IL5RA during the progression of pulmonary fibrosis.

2021年9月23日 · Regulation of the IL-5 receptor alpha (IL5RA) gene is complicated, with two known promoters (P1 and P2) driving transcription, and two known isoforms (transmembrane and soluble) dichotomously...

2005年12月1日 · Our findings suggest that polymorphisms in IL5RA might be among the genetic risk factors for asthma development, especially in atopic populations. IL5RA variant/haplotype information identified...

2021年7月11日 · Results: IL-5Rα expression on eosinophils inversely correlated with EO (r = -0.48; P < .0001), whereas serum levels of sIL-5Rα increased with the eosinophil count (r = 0.56; P < .0001) and serum IL-5 (r = 0.40; P < .0001) and IL-13 (r = 0.29; P = .004) levels.

Interestingly, the IL‐5Rα level responds differently to exposure to these cytokines depending on maturation state along the eosinophilopoietic pathway: in early CD34+ eosinophil progenitors (EoP), IL‐5 and IL‐ 9 upregulate IL‐5Rα mRNA and surface protein expression [18,20], while in mature pe‐ ripheral blood eosinophils, IL‐3, IL ...