2007年11月1日 · In this protocol we describe the in situ PCR method for the amplification of both DNA and mRNA targets [in situ reverse transcriptase-PCR (RT-PCR)], from frozen or paraffin-fixed tissue...

In situ PCR is a new molecular technique, that combines the extreme sensitivity of PCR with the cellular localization provided by in situ hybridization (ISH), through the amplification of specific gene sequences within intact cells or tissue sections and …

“The in situ PCR or slide PCR is a method used to amplify the DNA present in the paraffin-embedded tissue or formalin-fixed tissue on the glass slide.” In the traditional PCR or in the conventional PCR method, the DNA is amplified once it is extracted from the sample.

2014年9月18日 · In situ PCR is a relatively simple and rapid method that involves the amplification of specific mRNA directly within plant tissue whilst incorporating labelled nucleotides that are subsequently detected by immunohistochemistry. Another notable advantage of this technique is that it can be used on plants that are not easily genetically transformed.

The in-situ polymerase chain reaction (in-situ PCR) is a novel molecular technique that combines the extreme sensitivity of the PCR with the anatomical localization provided by in-situ hybridization.

2007年11月1日 · In this protocol we describe the in situ PCR method for the amplification of both DNA and mRNA targets [in situ reverse transcriptase- PCR (RT-PCR)], from frozen or paraffin-fixed tissue sections, cell culture or other single-cell suspensions.

1999年1月1日 · In situ polymerase chain reaction (IS-PCR) is a collective term used to describe primer-driven amplification of a DNA or RNA (IS- RTPCR) template by PCR and its subsequent detection within the confines of a histological tissue section or cell preparation.

Experimental protocols for successful in situ PCR share a number of important key steps. These include fixation and permeabilization during sample preparation, a mechanism for thermal cycling cellular material in solution or on glass slides, and a means to detect the amplificants (18).

Many groups have now published data based on the in situ detection of PCR-amplified DNA and cDNA. As with standard in situ hybridization or PCR, variables that can affect in situ PCR results include type of fixative and time of fixation, protease digestion, and the composition of the amplifying solu …

2004年1月1日 · Reverse transcriptase in situ polymerase chain reaction allows the direct cellular localization of any, including low-copy, RNA targets and requires several controls to document that nonspecific DNA synthesis pathways have been eliminated.