Genetically encoded fluorescent sensors reveal dynamic ... - Nature
2017年6月5日 · Upon NADPH binding, iNap1 showed a 3.5-fold increase and 2.5-fold decrease in fluorescence excited at 420 nm and 485 nm, respectively , leading to a 900% ratiometric …
NADPH metabolism determines the leukemogenic capacity and …
2022年4月5日 · The iNap1-high AML cells have enhanced proliferation capacities both in vitro and in vivo and are enriched for more functional leukemia-initiating cells than iNap1-low …
iNap1 can precisely indicate the NADPH level of AML
Moreover, we have confirmed NADPH level in iNap1-low and iNap1-high cells and demonstrated that iNap1-high cells indeed had much higher levels of NADPH as measured by enzyme …
iNap1 - NADPH Fluorescent Biosensor Details
T-Rex (78-189) SRSAQ pos 112-116 | SAG Linker | cpYFP | G Linker | T-Rex (190-211)
NADPH metabolism determines the leukemogenic capacity and …
2022年4月5日 · Using a highly sensitive, genetically encoded fluorescent sensor of nicotinamide adenine dinucleotide phosphate (NADPH), iNap1, we find three heterogeneous …
Analysis of redox landscapes and dynamics in living cells and in …
2018年9月26日 · To measure NADPH, NADH, and thiol redox in single living cells simultaneously, we expressed iNap1 in the cytosol, using a nucleus-excluded signal peptide, SoNar, in the …
Genetically encoded fluorescent sensors reveal dynamic regulation …
(f) Fluorescence intensity of iNap1 when excited at 420 nm plotted against the NADPH concentration at the indicated pH. (g) Kinetics of fluorescence response of purified iNap1 and …
Illuminating NAD+ Metabolism in Live Cells and In Vivo Using a ...
2020年4月20日 · We then study effects of macrophage activation on NADPH using the NADPH sensor iNap1 (Tao et al., 2017). LPS and IFN-γ treatment induced a significant decrease of …
【创新前沿】系列遗传编码荧光探针“精准追踪”细胞代谢
2021年11月18日 · 华理药学院杨弋教授团队，十余年来致力于生物化学与细胞生物学新方法领域的研究，领衔的“活细胞氧化还原代谢精准监测方法研究”项目，原创开发了Frex、SoNar、iNap …
iNap NADPH 传感器 – FR Biotechnology
表达 iNaps 的预制质粒，对 NADPH 具有高响应度的传感器。iNaps 的变体具有不同的亲和力，使其可用于具有各种 NADPH 水平的细胞或亚细胞器。 1\iNap传感器。原始的iNap传感器在420 …